Cbl Associates with Pyk2 and Src to Regulate Src Kinase Activity ...
All other coverslips were permeabilized in 0.05% saponin for 30 min. ... for 20 min, washed several times with PBS, and mounted in FluorSave (Calbiochem). ...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193648/...
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Specific Entry of Helicobacter pylori into Cultured Gastric ...
... by Paul Manning (University of Adelaide, Adelaide, South Australia, Australia). ... with 0 to 300 μM sodium ortho-vanadate (Calbiochem, Bad Soden,
Germany), ... and then incubated with 1 ml of 0.1% saponin in PBS at 37°C for 15
min. ...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=12...
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Characterization of a Nonclathrin Endocytic Pathway: Membrane ...
... from Chemicon (Melbourne, Australia) and R-phycoerythrin–conjugated anti- CD59 was from Leinco (St. Louis, MO). ... MO) and Biotin-X-NHS was from
Calbiochem (San Diego, CA). .... 0.2% saponin, and 2 mM final concentration of
filipin. ...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC491817/...
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The Gtpase Rho Controls a P53-Dependent Survival Checkpoint during ...
All washes after permeabilization were performed in saponin wash buffer (0.1% ... After washing, cells were incubated with 7AAD (Calbiochem-Novabiochem) for
...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=18...
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Insulin Increases Cell Surface GLUT4 Levels by Dose Dependently ...
... Australia), insulin was from Calbiochem (San Diego, Calif. ... min with 5% normal swine serum in the absence or presence of 0.1% saponin to analyze the ...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC434240/...
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Differences in the Frequency of Cytokine-Producing Cells in ...
Monensin (1 μM; Calbiochem) was included in all the cultures to inhibit ... in 50 μl of permeabilization buffer (PBS–1% FCS–0.1% sodium azide–0.1% saponin).
...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=10...
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N-WASP and cortactin are involved in invadopodium-dependent ...
4Australian Centre for Microscopy and Microanalysis Electron Microscope Unit, .... 0.1% glutaraldehyde, 0.15 mg/ml saponin in PBS, followed by treatment with
2 mg/ml NaBH4 in ... Phalloidin (Calbiochem) was used to stain for F-actin. ...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747479/...
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The mitochondrial protein Bak is pivotal for gliotoxin-induced ...
7John Curtin School of Medical Research, Australian National University, ..... or MnTBAP (Calbiochem) were added as described previously (Pardo et al., 2004).
... After two washes with 0.1% saponin in PBS, the cells were incubated with ...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=20...
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Progressive resistance of BTK-143 osteosarcoma cells to Apo2L ...
... ZVAD-fmk and ZDEVD-fmk were purchased from Calbiochem (Alexandria, NSW, Australia). .... and permeabilised with 0.1% saponin in PBS containing 10% ...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2394221/...
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Semen activates the female immune response during early pregnancy ...
Monensin (Calbiochem, La Jolla, CA; 2 μm) was added to all cultures to inhibit ..... This study was supported by the NHMRC of Australia and the Swedish Royal
Academy .... by immunofluorescence and the paraformaldehyde-saponin precedure.
...
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=17...
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