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14:42 EST 3rd December 2016 | BioPortfolio

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Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a p...

Augmenting Chinese hamster genome assembly by identifying regions of high confidence

Chinese hamster Ovary (CHO) cell lines are the dominant industrial workhorses for therapeutic recombinant protein production. The availability of genome sequence of Chinese hamster and CHO cells will ...

CHO and E. coli Residual DNA Quantification Kit

Product ReleaseBio-Rad’s new ddPCR CHO Residual DNA Quantification Kit and PCR E. coli Residual DNA Quantification Kit allow for direct quantification of host-cell DNA impurities in biopharmace...

#news #biotech New computational model provides a tool for improving the production of valuable drugs

// An extensive study involving partners from five continents has resulted in a model describing the metabolism of Chinese hamster ovary cells (CHO). This model can be used to improve and accelerate t...

Optimizing Protein Expression

Like a train that consists of a particular sequence of cars, from locomotive to caboose, proteins can only “leave the station” after each of its segments, amino acids, are hooked togethe...

Novel Genetically Engineered CHO Cells

Chinese hamster ovary (CHO)-based animal component-free (ACF) systems are currently responsible for producing more than $50 billion of therapeutic products a year. The introduction of animal origin-...

Biogen, Gates seek new antibody manufacturing techniques

Biogen Inc. (NASDAQ:BIIB) said it will co-fund a project with the Bill & Melinda Gates Foundation to improve production and manufacturing techniques for antibody-based therapies. Biogen said the initi...

Pharmaceutical Research: Targeted Delivery of Epirubicin to Cancer Cells by Polyvalent Aptamer System in vitro and in vivo

Type: Original PaperPurposeThe clinical use of Epirubicin (Epi), as an anthracycline drug, is limited because of its cardiotoxicity. Here, an Epirubicin (Epi)-modified polyvalent aptamer system (MPAS)...

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Chemopreventive effect of Toona sinensis leaf extract on 7,12-dimethylbenzaanthracene-induced hamster buccal pouch squamous cell carcinogenesis.

Toona sinensis leaf extract (TSL) has been shown to have anti-tumor effects on cancer cell lines. This study aimed to investigate the chemopreventive potential and the underlying mechanism of TSL duri...

Imaging visceral leishmaniasis in real time with golden hamster model: Monitoring the parasite burden and hamster transcripts to further characterize the immunological responses of the host.

Characterizing the clinical, immunological and parasitological features associated with visceral leishmaniasis is complex. It involves recording in real time and integrating quantitative multi-paramet...

A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction.

Chinese Hamster Ovary (CHO) cells are the current industry standard for production of therapeutic monoclonal antibodies at commercial scales. Production optimisation in CHO cells hinges on analytical ...

Antibacterial and Solubility Optimization of Thiomuracin A.

Synthetic studies of the antimicrobial secondary metabolite thiomuracin A (1) provided access to analogs in the Northern region (C2-C10). Selective hydrolysis of the C10 amide of lead compound 2 and s...

Seasonal differential expression of KiSS-1/GPR54 in the striped hamsters (Cricetulus barabensis) among different tissues.

Kisspeptins and G protein coupled receptor (GPR54) play significant roles in regulating reproductive activity among seasonal reproductive animals; however, the mechanisms of KiSS-1 and GPR54 gene affe...

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