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10:29 EDT 26th May 2016 | BioPortfolio

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Increase CHO Antibody Production Rapidly And Efficiently

Chinese Hamster Ovary (CHO) cells are used extensively to produce recombinant proteins. One of the most challenging aspects of culturing recombinant CHO cell clones is providing the diverse nutritiona...

A novel regulatory element (E77) isolated from CHO‐K1 genomic DNA enhances stable gene expression in Chinese hamster ovary cells

Vectors flanked by regulatory DNA elements have been used to generate stable cell lines with high productivity and transgene stability; however, regulatory elements in Chinese hamster ovary (CHO) cell...

Mechanisms underlying epigenetic and transcriptional heterogeneity in Chinese hamster ovary (CHO) cell lines

Recombinant cell lines developed for therapeutic antibody production often suffer instability or lose recombinant protein expression during long-term culture. Heterogeneous gene expression among cell ...

Survival of the oldest

The oldest surviving species of vertebrates, such as the cane toad and the California sea lion, which have endured past extreme environmental events, will be more likely to adapt to future climate cha...

Lonza Launches PowerCHO Advance™ Media for Improved Protein-Production Yields

Lonza has launched PowerCHO Advance™ Media, the latest addition to the company’s range of specialized, chemically defined, serum-free media for culturing Chinese Hamster Ovary (CHO) cells....

Lonza PowerCHO Advance Media

Lonza has launched PowerCHO Advance Media, the latest addition to the company’s range of specialised, chemically defined, serum-free media for culturing Chinese hamster ovary (CHO) cells. The media...

Hyperosmotic stimulus study discloses benefits in ATP supply and reveals miRNA/mRNA targets to improve recombinant protein production of CHO cells

Biopharmaceuticals are predominantly produced by Chinese hamster ovary (CHO) cells cultivated in fed‐batch mode. Hyperosmotic culture conditions (≥ 350 mOsmol kg‐1) resulting from feeding of nut...

Real‐time monitoring of specific oxygen uptake rates of embryonic stem cells in a microfluidic cell culture device

Oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. Quantification of cellular oxygen kinetics, i.e. the determination of specific oxyge...

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Photoperiod regulates the differential expression of KiSS-1 and GPR54 in various tissues and sexes of striped hamster.

In order to investigate how photoperiod affects the expression of KiSS-1/G-protein-coupled receptor 54 (GPR54), the hypothalamus, the ovaries and testicles of the striped hamster were studied followin...

Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites.

The grey hamster has been used in biomedical research for decades. However, effective molecular methods for evaluating the genetic structure of this species are lacking, which hinders its wider usage....

A Hamster Model of Diet-Induced Obesity for Preclinical Evaluation of Anti-Obesity, Anti-Diabetic and Lipid Modulating Agents.

Unlike rats and mice, hamsters develop hypercholesterolemia, and hypertriglyceridemia when fed a cholesterol-rich diet. Because hyperlipidemia is a hallmark of human obesity, we aimed to develop and c...

Hamster weight patterns predict the intensity and course of Schistosoma haematobium infection.

Although Syrian golden hamsters are a widely used host for experimental infection by Schistosoma haematobium, surprisingly little is known about the course of infection and associated intensity (as de...

Quantitative differentiation of normal and scarred tissues using second-harmonic generation microscopy.

The aim of this study was to differentiate normal and scarred hamster cheek pouch samples by applying a quantitative image analysis technique for determining collagen fiber direction and density in se...

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