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DAVIS SEQUENCING, INC. Company Profile

19:27 EST 23rd November 2017 | BioPortfolio

We offer DNA sequencing of single stranded or double stranded DNA samples from purified plasmids and PCR products. Our sequencing reactions are performed using the Applied Biosystems Big Dye Terminator V3.0 sequencing chemistry. One sequencing reaction consists of one DNA template (customer supplied plasmid DNA or PCR product) being sequenced with one primer (customer supplied primer or one of our supplied primers). We supply several frequently used sequencing primers for free, but the template DNA samples are supplied by our customers.
Our sequencing reaction read-lengths are normally between 800 and 900 bases (with a basecalling accuracy >99%) when run on our ABI 3730 DNA sequencers. Sequencing read-lengths are dependent upon several factors including template DNA concentration, purity and base composition (poly A and/or T regions, GC content and short tandem repeats) as well as primer binding specificity.

Since DNA sample purity and concentration are the two most critical factors to the success of automated sequencing reactions, we highly recommend that customers use the QIAGEN® plasmid DNA prep kits to purify their plasmid DNA samples. For purifying PCR products, we recommend using either the QIAquick Gel Extraction Kit (supplied by QIAGEN®) or Centricon® 100 columns (supplied by Amicon®) to remove unincorporated dNTPs and primers. In our experience, other DNA purification kits and methods are less reliable for preparing automated sequencing quality DNA samples. Please call us if you have any questions regarding the compatibility of other DNA sample preparation methods with our DNA sequencers.

Location

1490 Drew Avenue, Suite 170
Davis
California
95616
United States of America

Contact

Phone: (530) 297-5038
Fax: (530) 297-5039
Email: info@davissequencing.com


News Articles [488 Associated News Articles listed on BioPortfolio]

University of California Davis, Tempus Partner on Personalized Cancer Initiative

University of California Davis Comprehensive Cancer Center is collaborating with technology company Tempus on a precision medicine partnership. The new collaboration will focus on advancing clinical c...

Tempus, U of California Davis ink personalized medicine partnership

Tempus, a technology company focused on helping doctors personalize cancer care by collecting and analyzing large volumes of molecular and clinical data, and University of California Davis Comprehensi...

Author Raymond Davis’ Newly Released “Jesus Confirmed Raymond Davis 100% Proof” Recounts the Author’s Divine Encounter, which Reaffirmed his Faith

(PRWEB) July 14, 2017 “Jesus Confirmed Raymond Davis 100% Proof”: a confirmation of the Lord Jesus Christ. “Jesus Co...

UC Davis, Leibniz Institute for Photonic Technologies partner in biophotoni

(University of California - Davis) UC Davis and the Leibniz Institute for Photonic Technologies (Leibniz-IPHT), in Jena, Germany, have entered an agreement of cooperation to establish the Jena-Davis ...

UC Davis genome researchers facilitating NIH data commons pilot

(University of California - Davis) Researchers at the UC Davis School of Veterinary Medicine and Genome Center are taking part in an ambitious NIH initiative to make it easier for scientists to share ...

Next Gen Sequencing Challenges

Next generation sequencing opened the doors to our genome. It gives massive amounts of information in a week – whereas Sanger sequencing takes thrice as long, and causes lab lesions due to the abusi...

The Good, the Bad and the Expensive of Whole Genome Sequencing

Whole Genome Sequencing (WGS) is still very cutting edge, sequencing technology and while there are a lot of perks to using it, there are also a few drawbacks. The good, the bad and the pricey are out...

$1.6M from NSF to UC Davis to study water, land use in disadvantaged communities

(University of California - Davis) The National Science Foundation has awarded $1.6M to the University of California, Davis to analyze the complex relationships between surface water and groundwater s...

PubMed Articles [688 Associated PubMed Articles listed on BioPortfolio]

2017 Elda E. Anderson Award - Jason Davis.

Correction for Xu and Davis, "c-Jun NH2-Terminal Kinase Is Required for Lineage-Specific Differentiation but Not Stem Cell Self-Renewal".

DNA sequencing at 40: past, present and future.

This review commemorates the 40th anniversary of DNA sequencing, a period in which we have already witnessed multiple technological revolutions and a growth in scale from a few kilobases to the first ...

Whole exome sequencing in inborn errors of immunity: use the power but mind the limits.

Next-generation sequencing, especially whole exome sequencing (WES), has revolutionized the molecular diagnosis of inborn errors of immunity. This review summarizes the generation and analysis of next...

Correction to "Diverting Reactive Intermediates Toward Unusual Chemistry: Unexpected Anthranil Products from Davis-Beirut Reaction".

Clinical Trials [338 Associated Clinical Trials listed on BioPortfolio]

Enabling Personalized Medicine Through Exome Sequencing in the U.S. Air Force

The MilSeq Project is a nonrandomized, prospective pilot study of whole exome sequencing (WES) in the U.S. Air Force. The purpose of this study is to explore the implementation of WES into...

Bioequivalence Study of Gabapentin 800 mg Tablets Under Fed Conditions

This study compared the relative bioavailability (rate and extent of absorption) of Gabapentin tablets 800 mg by Ranbaxy Laboratories Limited with that of Neurontin ® 800 mg tablets of Pa...

Bioequivalence Study of Gabapentin 800 mg Tablets Under Fasting Conditions

This study compared the relative bioavailability (rate and extent of absorption) of the test Gabapentin tablets 800 mg by Ranbaxy Laboratories Limited to the reference Neurontin ® 800 mg ...

Genomic Sequencing in Patients With HCM Undergoing Septal Myectomy

Investigators aim to use comparative exome and/or genome sequencing to discover causative molecular lesions for phenotypes hypothesized to be caused by somatic mutations. For this study, i...

Genomic Sequencing in Acutely Ill Neonates

The purpose of this study is to compare the effectiveness of rapid next generation sequencing (NGS, such as whole genome sequencing1) with current practice to provide diagnostic or prognos...

Companies [242 Associated Companies listed on BioPortfolio]

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Nucleics was founded in 1999 by Dr Paul March and Dr Daniel Tillett to develop the ASIN genome sequencing technology. Nucleics is focused on the development of automated DNA sequencing services, reage...

Davis Vision

Headquartered in Plainview, N.Y., Davis Vision is one of America's leading vision benefits companies, providing comprehensive vision benefits for nearly 50 years. More than 55 million individuals nati...

Davis Phinney Foundation

The Davis Phinney Foundation was created in 2004 by cycling legend Davis Phinney. Davis Phinney remains the race leader in victories by an American cyclist, and he is a two-time Tour de France stage w...

DAVIS SEQUENCING, INC.

We offer DNA sequencing of single stranded or double stranded DNA samples from purified plasmids and PCR products. Our sequencing reactions are performed using the Applied Biosystems Big Dye Terminato...

Davis Vision, Inc.

Davis Vision is part of the HVHC Inc. group of companies, which also includes Viva International Group and Eye Care Centers of America. One of the nation's premier managed vision care companies, Davis...

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DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...

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Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...


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