Diagnostics Research

02:47 EDT 19th September 2018 | BioPortfolio

An observational study of environmental exposures, airway cytology, and performance in racing thoroughbreds.

Mild equine asthma is presumed to arise in response to environmental exposures but the relative impact of differing inflammatory phenotypes upon performance are largely unexplored.

Species Differentiation and Quantification of Processed Animal Proteins and Blood Products in Fish Feed using an 8-plex Mass Spectrometry-Based Immunoassay.

With the reintroduction of non-ruminant processed animal proteins (PAPs) for the use in aquaculture in 2013, there is a suitable alternative to replace expensive fish meal in fish feed. Nevertheless, since the bovine spongiform encephalopathy (BSE) crisis, the use of PAPs in feed is strictly regulated. To date, light microscopy and polymerase chain reaction are the official methods for proving the...

Identification of insulin-regulated aminopeptidase (IRAP) in the rat pineal gland and the modulation of melatonin synthesis by angiotensin IV.

A local renin-angiotensin system (RAS) has been postulated in the pineal gland. In addition to angiotensin II (Ang II), other active metabolites have been described. In this study, we aimed to investigate a role for Ang IV in melatonin synthesis and the presence of its proposed (IRAP)/AT receptor (insulin-regulated aminopeptidase) in the pineal gland. The effect of Ang IV on melatonin synthesis wa...

Non-O157 Shiga Toxin-Producing Escherichia coli - a Poorly Appreciated Enteric Pathogen: Systematic Review.

Non-O157 strains of STEC are more common causes of acute diarrhea than the better-known O157 strains and have the potential for large outbreaks. This systematic review of the literature identified 129 serogroups as well as 262 different O and H antigen combinations of Shiga-toxin-producing Escherichia coli (STEC) in cases of epidemic and sporadic disease worldwide. Removing results from a single l...

Comparative performance of human papillomavirus messenger RNA versus DNA screening tests at baseline and 48 months in the HPV FOCAL trial.

HPV FOCAL is a randomized trial comparing high-risk HPV [Hybrid Capture 2 (HC2)] vs. liquid-based cytology (LBC) for primary cervical screening.

In Silico Identification and in Vitro Analysis of B and T-Cell Epitopes of the Black Turtle Bean (Phaseolus Vulgaris L.) Lectin.

The incidence of lectin allergic disease is increasing in recent decades, and definitive treatment is still lacking. Identification of B and T-cell epitopes of allergen will be useful in understanding the allergen antibody responses as well as aiding in the development of new diagnostics and therapy regimens for lectin poisoning. In the current study, we mainly addressed these questions.

Comparison of early and recent influenza A(H1N1)pdm09 isolates harboring or not the H275Y neuraminidase mutation, in vitro and in animal models.

After 6 years of circulation in humans, a novel antigenic variant of influenza A(H1N1)pdm09 (i.e., A/Michigan/45/2015) emerged in 2015-16 and has predominated thereafter worldwide. Herein, we compared in vitro and in vivo properties of 2016 wild-type (WT) A/Michigan/45/15-like isolate and its H275Y neuraminidase (NA) variant to the original A/California/07/09-like counterparts. The H275Y mutation ...

Rapid quantification of vesicular stomatitis virus in Vero cells using Laser Force Cytology.

The ability to rapidly and accurately determine viral infectivity can help improve the speed of vaccine product development and manufacturing. Current methods to determine infectious viral titers, such as the end-point dilution (50% tissue culture infective dose, TCID50) and plaque assays are slow, labor intensive, and often subjective. In order to accelerate virus quantification, Laser Force Cyto...

Salivary C-reactive protein among at-risk adolescents: A methods investigation of out of range immunoassay data.

Inflammatory markers including C-Reactive Protein (CRP) are increasingly used within research and clinical settings. Yet, varying methodologies for cleaning immunoassay data with out of range (OOR) samples may alter characteristic levels of CRP, thereby obscuring interpretation and reliability. This study investigated the influence of eight immunoassay OOR data treatment techniques on salivary CRP...

Removal of Bilirubin with a New Adsorbent System: In Vitro Kinetics.

Many potentially toxic molecules accumulate in the blood during hepatic dysfunction. In clinical practice, it is very difficult to remove bilirubin, the most widely studied toxin, and particularly the unconjugated form, strongly albumin-bound. The aim of this in vitro study was to assess irreversible bilirubin adsorption as a protein-bound compound marker, using Cytosorb® (Cytosorbents Corp.), a ...

Sensitivity and specificity of a lateral flow immunoassay (LFI) in serum samples for diagnosis of melioidosis.

Culture is the gold standard for the diagnosis of melioidosis, an infection caused by Burkholderia pseudomallei. Here we evaluate a lateral flow immunoassay (LFI) to detect B. pseudomallei capsular polysaccharide (CPS) in serum samples.

Rare-earth fluorescence thermometry of laser-induced plasmon heating in silver nanoparticles arrays.

The laser-induced plasmon heating of an ordered array of silver nanoparticles, under continuous illumination with an Ar laser, was probed by rare-earth fluorescence thermometry. The rise in temperature in the samples was monitored by measuring the temperature-sensitive photoluminescent emission of a europium complex (EuTTA) embedded in PMMA thin-films, deposited onto the nanoparticles array. A max...

Graphene oxide wrapped with gold nanorods as a tag in a SERS based immunoassay for the hepatitis B surface antigen.

A composite consisting of graphene oxide and gold nanorods (GO-GNRs) was designed for the trace determination of hepatitis B surface antigen (HBsAg) using surface enhanced Raman spectroscopy (SERS). GO contains numerous carboxy and hydroxy groups on its surface and therefore can serve as the substrate for decoration with GNRs and for immobilizing antibody against HBsAg. The GNRs (carrying the SERS...

Systematic review of pre-clinical chronic myeloid leukaemia.

Studies of a provisional entity pre-clinical chronic myeloid leukaemia (CML), which precedes chronic phase (CP) without leucocytosis or blood/marrow feature of CML CP, has been increasing.

Ultrasound diagnostics for myotendinous injuries and insertion tendinopathies of the elbow joint.

Acute capsular injuries of the elbow joint and its ligaments predominantly occur after joint dislocation or subluxation. Together with lateral and medial epicondylitis they represent a common injury pattern in radiology, orthopedic and trauma surgery.

The "American (Cancer) Patients First" Plan to Reduce Drug Prices-A Critical Assessment.

An unanticipated finding on peripheral smear: Blue-green crystals of impeding death?

Impact of Clone Size With a Single Cytogenetic Abnormality on the Revised International Prognostic Scoring System in Myelodysplastic Syndromes.

Differential diagnosis of mesenchymal neoplasms of the digestive tract by cell block and immunohistochemistry.

To evaluate the diagnostic yield of the cell block (CB) technique with immunohistochemistry in patients with mesenchymal neoplasms of the gastrointestinal tract collected by endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA).

Defining reference intervals for a serum growth differentiation factor-15 (GDF-15) assay in a Caucasian population and its potential utility in diabetic kidney disease (DKD).

Growth differentiation factor-15 (GDF-15), a stress responsive cytokine, is a promising biomarker of renal functional decline in diabetic kidney disease (DKD). This study aimed primarily to establish normative data and secondarily to evaluate the potential utility of GDF-15 in DKD using Roche Diagnostics electrochemiluminescence immunoassay (ECLIA) in an Irish Caucasian population.

Clinico-cytopathologic spectrum, including uncommon forms, of nine cases of chordomas with immunohistochemical results, including brachyury immunostaining: a single institutional experience.

To present clinical and cytopathologic features of 9 cases of chordomas, diagnosed over 9 years' duration and confirmed by brachyury (T) immunostaining.

A Fond Salute to George Stamatoyannopoulos - Scholar Leader Mentor Colleague Friend.

Effect of setting atmosphere on apatite cement resorption: An in vitro and in vivo study.

The aim of this present study was to investigate the effect of setting atmosphere on replacement of apatite cement with new bone both in vitro and in vivo.

Carcinocythemia: A rare entity becoming more common? A 3-year, single institution series of seven cases and literature review.

Carcinocythemia is a rare phenomenon defined as morphologically identifiable, circulating tumor cells in the peripheral blood. No modern case series of carcinocythemia exists in the literature.

Tunable Fluorescence from A Responsive Hyperbranched Polymer with Spatially Arranged Fluorophore Array.

The present study constructed a water-soluble hyperbranched polymer platform that contained Förster resonance energy transfer (FRET) array and exhibited varied fluorescence in response to solvent, light and CN- anion stimuli. The use of chain-growth copper-catalyzed azide-alkyne cycloaddition polymerization (CuAACP) enabled accurate control of the ratio and distance of three incorporated fluoroph...

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