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Protein Structures Clarified by Using Novel Technique

05:06 EDT 5 Jul 2017 | Genetic Engineering News

Protein Structures Clarified by Using Novel Technique Researchers at the Salk Institute report that tilting a sheet of frozen proteins--by anywhere from 10 to 50 degrees--as it lies under the microscope gives higher quality data and could lead to a better understanding of a variety of diseases. "People have tried to implement tilting before, but there have been a lot of challenges," says Dmitry Lyumkis, Ph.D., a Helmsley-Salk Fellow at the Salk Institute and senior author of the new paper (“Addressing preferred specimen orientation in single-particle cryo-EM through tilting”), published in Nature Methods . "We've eliminated many of these problems with our new approach." “We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection,” write the investigators. “We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes.” Cryo-EM, ...

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