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Enzyme-linked immunosorbent assays (ELISAs) represent the gold standard for quantitatively detecting protein biomarkers and are used for applications such as diagnosing or monitoring disease, developing therapeutic approaches, tracking patients’ responses to treatment, and evaluating patients for clinical trials. But ELISAs are not particularly user friendly. The tests are time consuming and resource intensive and require trained personnel or automated liquid-handling robots, together with bulky data analysis equipment. ELISAs may also require multiple blocking and washing steps to prevent unwanted binding by nonspecific proteins that create background noise and reduce sensitivity. All these factors hold back the use of ELISAs in limited-resource settings and can delay results reaching clinicians who might be waiting to make life-saving decisions on treatment, reports a team of researchers at Duke University, Durham, NC. The Duke team, headed by Ashutosh Chilkoti, Ph.D., Daniel Y. Joh, and Angus M. Hucknall, Ph.D., has now developed a ...NEXT ARTICLE
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...
Immunoassay - ELISA
Immunoassays are quick and accurate tests to detect specific molecules. Immunoassays rely on an antibody to bind to the specific structure of a molecule. Antibodies are proteins generated by animals in response to the invasion of a foreign molecule (anti...
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...