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Cofactor engineering for enhanced production of diols by Klebsiella pneumoniae from co‐substrate

00:38 EDT 19 Aug 2017 | Wiley Biotechnology Journal

Diols, such as 1,3‐propanediol (1,3‐PDO) and 2,3‐butanediol (2,3‐BDO), have several promising properties for many synthetic reactions. Here, the cofactor engineering strategy, including the construction of Entner‐Doudoroff pathway and transhydrogenase‐based NADH regeneration system, was applied in producing diols from mixtures of glucose and glycerol. Entner‐Doudoroff pathway had a high regeneration rate of NAD(P)H. This work described a strategy to administrate intracellular NADH/NAD+ ratio and improved the concentration of diols. The improvement of NADH/NAD+ ratio also effected gene transcription level of the central carbon pathway and cell growth. Finally, the intracellular NADH/NAD+ ratio in KP‐APZDUT was increased by 92.8% compared to the KP‐T and the concentration, yield and productivity of diols were increased to 110.8 g/L, 0.78 mol/mol and 3.46 g/Lh, respectively. The strategy described here provides an approach to achieve a recombinant strain which is capable of producing diols with high yield and productivity. To the best of our knowledge, the Entner‐Doudoroff pathway has not yet been used to produce 1,3‐PDO or 2,3‐BDO in Klebsiella pneumoniae.

Original Article: Cofactor engineering for enhanced production of diols by Klebsiella pneumoniae from co‐substrate

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