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Strain breeding enhanced heterologous cellobiohydrolase secretion by Saccharomyces cerevisiae in a protein specific manner

00:44 EDT 20 Aug 2017 | Wiley Biotechnology Journal

The yeast Saccharomyces cerevisiae has a long association with alcoholic fermentation industries and has received renewed interest as a biocatalyst for secondā€generation bioethanol production. Rational engineering strategies have been used to create yeast strains for consolidated bioprocessing of lignocellulosic biomass. Although significant progress was made in this regard with the expression of different cellulolytic activities in yeast, cellobiohydrolase (CBH) titers remain well below ideal levels. Through classical breeding, we obtained S. cerevisiae strains with up to 2ā€fold increased CBH secretion titers in strains expressing a single gene copy. An increase of up to 3.5ā€fold in secreted cellobiohydrolase activity was subsequently shown for strains expressing the heterologous gene on a high copy episomal vector. To our knowledge, this is the first report of classical breeding being used to enhance heterologous protein secretion and also the most significant enhancement of CBH secretion in yeast yet reported. This enhanced secretion phenotype was specific for cellobiohydrolase I secretion, indicating that reporter protein properties might be a major determining factor for efficient protein secretion in yeast. By exploring the latent potential of different S. cerevisiae strains, we show that the allele pool of various strains is a valuable engineering resource to enhance secretion in yeast.

Original Article: Strain breeding enhanced heterologous cellobiohydrolase secretion by Saccharomyces cerevisiae in a protein specific manner

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