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Codon-optimization of HIV-1 Viral Infectivity Factor (VIF) Gene

19:00 EST 31 Dec 2003 | NIH

Expression of the HIV-1 Vif protein in the absence of other viral factors such a Tat and Rev is extremely inefficient due to the presence of inhibitory sequences on its mRNA. This invention uses codon optimization to remove such inhibitory sequences without altering the amino acid sequence of the protein. The modified vif gene in the resulting pcDNA -hVIF vector is expressed under the control of the CMV promoter. In this, the protein functions as wild type and is more amendable to high-level expression in mammalian cells.

Currently this vector is used in on-going studies of HIV infection and its ability to overcome cellular restriction to replication. As such, the reagent will be valuable to other researchers in discovering mechanisms of replication, next generation therapeutics and potentially prevention of infection as well.

IC: 
NIAID
NIH Ref. No.: 
E-041-2004/0
Applications: 
  • A plasmid pcDNA-hVif capable of expressing VIF independent of other HIV genes through removal of inhibitory sequences on its mRNA.
Provider Technology ID: 
846
Updated On: 
Sep 19, 2017
Date Published: 
Thursday, January 1, 2004
Provider Classifications: 
Publications: 
Patent Authority: 
EIR
Licensing Contacts: 
Lead Inventor: 
Inventor IC: 
NIAID
NIAID
NIAID
Inventor Lab URL: 
https://irp.nih.gov/pi/klaus-strebel
LPM FIrst Name: 
Vince
LPM Last Name: 
Contreras
LPM Address: 
5601 Fishers Lane, Suite 6D (MSC 9804)
LPM City: 
Rockville
LPM Zip: 
20892-9804
Inv Is lead: 
LPM State: 
MD
LPM Phone: 
240-669-2823
LPM Suffix: 
Ph.D.
LPM Organization: 
NIH Office of Technology Transfer
LPM Fax: 
240-627-3117
DTDT Classification: 
Devices/Instruments; Software; Mfg Technology/Methods; Research Tools & Material
Diagnostics
Infectious Diseases
Diagnostics
AIDS (only)
Research Materials
Infectious Diseases (viral)
Research Tool
DTDT Description: 
Devices/Instrumentation
Devices/Instrumentation - Diagnostics
Infectious Diseases
Infectious Diseases - Diagnostics
Infectious Diseases - Diagnostics - Viral: AIDS
Infectious Diseases - Research Materials
Infectious Diseases (viral)
Research Tool
Additional Patents: 
Research Material - Patent protection is not being pursued for this technology. (IC Reference No. 2004-036)
Application Term: 
Collaboration Sought: 
Yes
Institute or Center: 
E Number Only: 
E-041-2004
Inventor First Name: 
Klaus
Stephan
Kim-Lien
Inventor Last Name: 
Strebel
Bour
Nguyen

Original Article: Codon-optimization of HIV-1 Viral Infectivity Factor (VIF) Gene

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