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The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualising perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations.
The product has been validated in human peripheral blood monocytes and human and mouse monocyte/macrophage cell lines. Uptake is assessed via flow cytometry or fluorescence microscopy in the far-red channel. Phagocytosis is detectable in 1–4 h at 37°C.
The kit provides enough Latex Bead-Rabbit IgG-DyLight 633 Complex for up to 500 samples.
Original Article: Cayman Chemical Phagocytosis Assay Kit (IgG-DyLight 633)NEXT ARTICLE
An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity. This can be a drug or biochemical substance or a cell in an organism or organic sample. ...