South Bay Bio ubiquitination TR-FRET kits

19:00 EST 15 Nov 2017 | Australian Life Scientist

South Bay Bio’s real-time TR-FRET ubiquitin conjugation assays are simple: the format is 96- or 384-well low-volume plates and assays are ‘set and forget’.

A mixture of ubiquitins labelled with cryptate and acceptor fluorophore are combined with UBA1 activating enzyme, E2 conjugating enzyme, E3 ligase, substrate protein (optional) and 10x reaction buffer, and initiated with addition of ATP. As donor and acceptor ubiquitins become incorporated into chains they come into close proximity, resulting in energy transfer (FRET) and signal detection. Readout can be collected in real time, facilitating enzyme kinetics or endpoint if preferred. Assay performance is typically unparalleled: Z’ are usually >0.8 and signal-to-noise is commonly >3000%.

The kits enable the study of auto-ubiquitination kinetics for several human recombinant E3 ligases: MDM2, ITCH and Parkin. Coupled with the assay’s short development time (as no antibody development is required for assay optimisation), the assay platform is suitable for a wide variety of academic and industry screening applications.

Kit components are also available separately.

Original Article: South Bay Bio ubiquitination TR-FRET kits


More From BioPortfolio on "South Bay Bio ubiquitination TR-FRET kits"

Quick Search


Relevant Topics

Immunoassay - ELISA
Immunoassays are quick and accurate tests to detect specific molecules. Immunoassays rely on an antibody to bind to the specific structure of a molecule. Antibodies are proteins generated by animals in response to the invasion of a foreign molecule (anti...

Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...