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Parallel Measurements of Multiple Macromolecules Using a Cryoarray

19:00 EST 31 Dec 2001 | NIH

Available for license is a new improved technique for the creation of biological arrays of 25-100 biological samples per slide, for use in parallel molecular screening in medical research and clinical diagnostics. Recent advances in genomics, including serial analysis of gene expression, and DNA microarrays have allowed researchers to perform high throughput analysis of gene expression. These experiments generate large amounts of information that must be validated independently, one gene at a time. In particular, there is an increasing demand for protein arrays in order to measure changes in protein expression or post-translational modification of proteins. Current techniques to create protein arrays are deficient because the proteins stick to the arraying pins, and array fabrication at room temperature may destroy the protein structure and function. The CryoArray technology, based on the creation of the arrays at subzero temperature, preserves the stability and functionality of the biological samples, including proteins, and is flexible with respect to the molecular probes it can accommodate. Wells made in a frozen block of embedding material are filled with biological samples, which freeze and bond to the surrounding block. The loaded block is cut in a cryostat to produce up to 800 replicate 4-10 microns thin sections. The samples can include DNA, RNA, and proteins such as antibodies or receptors. Recombinant or native tissue proteins are detected using antibodies; however, the system can be extended for other types of biological assays.

The ability to make multiple (i.e., up to 800) cryosections from one cryoblock enables parallel analysis of many identical arrays. Unlike other proteomic techniques, cryoarrays are easy to use, economical, efficiently use samples with little waste, require only a small volume of sample, and are protein friendly because samples are kept frozen during production. The cryoarray method allows small laboratories without access to expensive arraying equipment to produce many identical arrays with moderate numbers of precious samples. Proteins can be detected in their native configuration, without SDS or formalin. Cryoarrays may be useful for screening small samples of precious biological fluids or tissues for new biomarkers or for rapid screening of monoclonal antibodies. It may be possible to use cryoarrays to also measure protein function and protein-protein interactions.

IC: 
NIDDK
NIH Ref. No.: 
E-064-2001-0
TAB No: 
TAB-544
Advantages: 
  • Preserves the stability and functionality of the biological samples being examined
  • The ability to make multiple cryosections from one cryoblock enables parallel analysis of many identical arrays
  • Easy to use and affordable system
Applications: 
  • Research involving molecular screening
  • Clinical diagnostics
Development Status: 

Late stage

Provider Technology ID: 
544
Updated On: 
May 15, 2018
Date Published: 
Tuesday, January 1, 2002
Provider Classifications: 
Publications: 
Patent Application: 
PCT/US02/27955
60/316,544
10/233,193
Patent Authority: 
PCT
US
US
2016-02-29 00:00:00
2016-02-29 00:00:00
Patent Number: 
6,951,761
Licensing Contacts: 
LPM Address: 
  --
United States
Lead Inventor: 
Inventor IC: 
NIDDK
NIDDK
NCI
NCI
Inventor Lab URL: 
http://irp.nih.gov/pi/robert-star
https://ccr.cancer.gov/laboratory-of-pathology/stephen-m-hewitt
LPM FIrst Name: 
Jaime
LPM Last Name: 
Greene
LPM Address: 
9609 Medical Center Drive, Room 1E-530 (MSC 9702)
LPM City: 
Rockville
LPM Zip: 
20850-9702
Inv Is lead: 
LPM State: 
MD
LPM Phone: 
240-276-6633
LPM Suffix: 
M.S.
LPM Organization: 
NIH Office of Technology Transfer
LPM Fax: 
240-276-5504
DTDT Classification: 
Devices/Instruments; Software; Mfg Technology/Methods; Research Tools & Material
Diagnostics
Devices (catheters, shunts, stents, other devices not quite instruments)
Research Tools and Materials
Instruments (e.g. spectrometers, microscopes)
Cancer
Diagnostics
Infectious Diseases
Diagnostics
Gene Based Therapies
Diagnostics
Internal Medicine
Diagnostics
DTDT Description: 
Devices/Instrumentation
Devices/Instrumentation - Diagnostics
Devices/Instrumentation - Diagnostics - Devices
Devices/Instrumentation - Research Tools and Materials
Devices/Instrumentation - Research Tools and Materials: Instruments
Cancer
Cancer - Diagnostics
Infectious Diseases
Infectious Diseases - Diagnostics
Gene Based Therapies
Gene Based Therapies - Diagnostics
Internal Medicine
Internal Medicine - Diagnostics
Pat Filing Date: 
2002-08-30
2001-08-31
2002-08-30
Patent Issue Date: 
2005-10-04
Application Term: 
Disease Area Term: 
Collaboration Sought: 
Yes
Institute or Center: 
E Number Only: 
E-064-2001-0
Inventor First Name: 
Robert
Takehiko
Stephen
Lance
Inventor Last Name: 
Star
Miyaji
Hewitt
Liotta

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