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Construction of an Infectious Full-Length cDNA Clone of the Porcine Enteric Calicivirus RNA Genome

20:00 EDT 31 May 2008 | NIH

Porcine enteric calicivirus (PEC) is a member of the genus Sapovirus in the family Caliciviridae. This virus causes diarrheal illness in pigs, and is presently the only enteric calicivirus that can be grown in cell culture. In addition to its relevance to veterinary medicine as a diarrheal agent in pigs, PEC serves as an important model for the study of enteric caliciviruses that cause diarrhea and that cannot be grown in cell culture (including the noroviruses represented by Norwalk virus). The development of an infectious cDNA clone is important because it enables the use of “reverse genetics” to engineer mutations of interest into the genome of PEC and to study their effects. In addition, it allows the introduction of foreign coding sequences into the genome of PEC that could be useful for vaccine development in swine and possibly humans. This discovery has both basic research applications such as mapping mutations involved in tissue culture adaptation, tissue tropism, and virulence as well as practical applications such as providing a genetic backbone for the development of chimeric vaccine viruses.

IC: 
NIAID
NIH Ref. No.: 
E-214-2003-0
Provider Technology ID: 
909
Updated On: 
May 16, 2018
Date Published: 
Sunday, June 1, 2008
Provider Classifications: 
Publications: 
Patent Authority: 
EIR
Licensing Contacts: 
Lead Inventor: 
Inventor IC: 
NIAID
NIAID
NIAID
NIAID
Inventor Lab URL: 
https://irp.nih.gov/pi/kim-green
LPM FIrst Name: 
Peter
LPM Last Name: 
Soukas
LPM Address: 
5601 Fishers Lane, Suite 6D (MSC 9804)
LPM City: 
Rockville
LPM Zip: 
20892-9804
Inv Is lead: 
LPM State: 
MD
LPM Phone: 
301-496-2644
LPM Suffix: 
J.D.
LPM Organization: 
NIH Office of Technology Transfer
LPM Fax: 
240-627-3117
DTDT Classification: 
Infectious Diseases
Diagnostics
Non-AIDS (only)
Therapeutics
Non-AIDS (only)
Vaccines
Non-AIDS (only)
Research Materials
DTDT Description: 
Infectious Diseases
Infectious Diseases - Diagnostics
Infectious Diseases - Diagnostics - Viral: Non-AIDS
Infectious Diseases - Therapeutics
Infectious Diseases - Therapeutics - Anti-virals: Non-AIDS
Infectious Diseases - Vaccines
Infectious Diseases - Vaccines - Viral: Non-AIDS
Infectious Diseases - Research Materials
Publication Link: 
https://www.ncbi.nlm.nih.gov/pubmed/12504571
Publication Caption: 
PMID 12504571
Publication Title: 

Chang KO, et al. Cell-culture propagation of porcine enteric calicivirus mediated by intestinal contents is dependent on the cyclic AMP signaling pathway.

Additional Patents: 
Research Material -- Patent protection is not being pursued for this technology
Disease Area Term: 
Collaboration Sought: 
Yes
Institute or Center: 
Collaboration Opportunity: 

The Laboratory of Infectious Diseases, NIAID, NIH, is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize reagents derived from an infectious cDNA copy of the genome of porcine enteric calicivirus. Please contact Kim Y. Green at kgreen@niaid.nih.gov for more information.

Related Patent Authority: 
EIR
EIR
EIR
Related Invention: 
E-198-2003-0
E-212-2003-0
E-283-2003-0
E Number Only: 
E-214-2003-0
Inventor First Name: 
Kyeong-Ok
Lisbeth Kim
Stanislav
Gael
Inventor Last Name: 
Chang
Green
Sosnovtsev
Belliot

Original Article: Construction of an Infectious Full-Length cDNA Clone of the Porcine Enteric Calicivirus RNA Genome

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