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Method to Remove Mycoplasma Contamination from Virus Stocks

20:00 EDT 12 Jun 2018 | NIH

Mycoplasma are a form of bacteria that are commonly found as contaminants in cell cultures. They adversely affect cell line growth rates and viral vaccine production. Mycoplasma contamination is a challenge for the vaccine industry and virology researchers. Current commercial reagents or kits only temporarily inhibit the growth of mycoplasma, but cannot eliminate the contaminants.

CDC has developed a method that can reliably eliminate mycoplasma from enveloped virus stock. This method allows for the removal of the mycoplasma without harming harvested virus. The harvested virus can then be transfected back into a clean cell line for continued virus production. Although designed for purification of rabies virus stocks, the method may successfully remove mycoplasma contamination from stocks of other enveloped viruses such as measles, mumps, respiratory syncytial virus, and influenza virus. Additional in vitro testing is planned for other virus purification. Rabies strains treated using this method are available.

IC: 
CDC
NIH Ref. No.: 
E-039-2018-0
Advantages: 
  • Permanent removal of mycoplasma
  • Increased cell growth rate and improved vaccine production
Applications: 
  • Restore a clean vaccine repository from historical old strains for vaccine research and development
  • A kit to selectively remove mycoplasma from virus seed stocks
  • Research tool for virus study
Provider Technology ID: 
3291
Updated On: 
Jun 13, 2018
Date Published: 
Wednesday, June 13, 2018
Provider Classifications: 
Publications: 
Patent Application: 
62/645,002
Patent Authority: 
US
Licensing Contacts: 
Lead Inventor: 
Inventor IC: 
CDC
CDC
CDC
LPM FIrst Name: 
Jeremiah
LPM Last Name: 
Mitzelfelt
LPM Address: 
BG 5601FL RM 2G29 MSC 9804 5601 FISHERS LN
LPM City: 
Bethesda
Inv Is lead: 
LPM Zip: 
20892-9804
LPM State: 
MD
LPM Phone: 
301-443-8518
LPM Organization: 
NIH Office of Technology Transfer
DTDT Classification: 
Infectious Diseases (viral)
Zoonotic/Veterinary
Diagnostic
Quality Control/Quality Assurance
Research Tool
Other (this will include others such as manufacturing, consumer products and vet
Vaccine
Vaccine
DTDT Description: 
Infectious Diseases (viral)
Zoonotic/Veterinary
Diagnostic
Quality Control/Quality Assurance
Research Tool
Other (this will include others such as manufacturing, consumer products and vet
Vaccine
Vaccine
Pat Filing Date: 
2018-03-19
Technology Term: 
Collaboration Sought: 
Yes
Institute or Center: 
Collaboration Opportunity: 

The CDC Technology Transfer Office (TTO) is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize: Method to Remove Mycoplasma Contamination from Virus Stocks.
For collaboration opportunities, please contact CDC TTO at tto@cdc.govor 1-404-639-1330.

Related Invention: 
E-040-2018-0
E-099-2018-0
E Number Only: 
E-039-2018-0
Inventor First Name: 
Xianfu
Victoria
Yong
Inventor Last Name: 
Wu
Olson
Yang

Original Article: Method to Remove Mycoplasma Contamination from Virus Stocks

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