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Software for Automated Generation of Density Maps

14:37 EDT 12 Jul 2018 | NIH

The  National Cancer Institute (NCI) is seeking parties interested in licensing software for the automated determination of macromolecular structures using cryo-electron microscopy.

The technology available is computer software for the automated generation of density maps of macromolecular structures from a series of 2D digital micrographs of frozen hydrated specimens collected using an electron microscope equipped with an ultra-cooled computerized stage.  A series of images of biological specimens collected at different tilt angles relative to the electron beam are aligned to compensate for mechanical errors of the stage and combined to obtain 3D images (tomograms). Sub-volumes containing a single macromolecular complex can be extracted from the 3D image of a protein solution, or from a suspension of viruses or cells. The individual sub-volumes of identical structures are aligned and averaged together to generate a density map of the macromolecular complex of interest.
 

NIH Ref. No.: 
E-162-2012
Advantages: 
  • Noise processing
  • Algorithmic averaging
Applications: 
  • Macromolecular imaging
  • Mapping of molecular interactions
  • Determination of molecular structure and reactivity
Development Status: 

Prototype

Updated On: 
Jun 7, 2018
Provider Classifications: 
Publications: 
Licensing Contacts: 
Lead Inventor: 
LPM FIrst Name: 
John
LPM Last Name: 
Hewes
Inv Is lead: 
LPM Phone: 
240-276-5515
LPM Suffix: 
Ph.D.
LPM Organization: 
NCI - National Cancer Institute
DTDT Classification: 
Software
DTDT Description: 
Software
Publication Link: 
https://www.ncbi.nlm.nih.gov/pubmed/17855124
https://www.ncbi.nlm.nih.gov/pubmed/16190467
Publication Caption: 
PMID 17855124
PMID 16190467
Publication Title: 

F. Amat et al. Markov random field based automatic image alignment for electron tomography. 

A. Bartesaghi et al. An energy-based three-dimensional segmentation approach for the quantitative interpretation of electron tomograms.

Technology Term: 
Collaboration Sought: 
Yes
Collaboration Opportunity: 

Licensing and research collaboration

E Number Only: 
E-162-2012
Inventor First Name: 
Mario
Inventor Last Name: 
Borgnia

Original Article: Software for Automated Generation of Density Maps

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