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Novel Plasmid Vectors for the Soluble Expression of Recombinant Proteins in Escherichia coli

19:00 EST 20 Feb 2013 | NIH

A series of novel plasmid vectors for the soluble expression and subsequent purification of recombinant proteins that have historically proven to be extremely difficult to purify from Escherichia coli (E. coli) are provided. Because of its ease of growth and generally low cost to cultivate, E. coli is often employed as the host for vectors expressing recombinant proteins. In an ideal situation, the recombinant protein is expressed from a strong promoter, highly soluble, and recovered in high yield and activity.

Original Article: Novel Plasmid Vectors for the Soluble Expression of Recombinant Proteins in Escherichia coli

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