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Real-time PCR Detection of Streptococcus pneumoniae with High Sensitivity and Specificity

20:00 EDT 9 Jul 2018 | NIH

Streptococcus pneumoniae em> is the leading cause of community-acquired pneumonia and is also a frequent cause of bloodstream, brain and spinal cord, ear, and sinus infections. According to 2015 CDC data, an estimated 900,000 Americans get pneumococcal pneumonia each year and approximately 5-7% die from it annually. Accurate diagnosis and early treatment are important for improving patient outcomes.

Pneumonia is typically diagnosed by clinical examination, chest X-rays, and culture of patient blood and secretions. X-rays cannot identify the pathogen; blood cultures take several days to grow with limited reliability; and sputum and throat culture specimens collected may not contain isolates of the organism. CDC researchers developed a Taqman-based assay that uses real-time PCR to detect three specific gene regions of S. pneumoniae em> with a very low limit of detection of 10 copies, and has shown high sensitivity and specificity against a panel of 67 CDC isolates. Targeting multiple genes, this technology would perform well in a multiplex diagnostic testing application.

IC: 
CDC
NIH Ref. No.: 
E-288-2013-0
Advantages: 
  • High throughput
  • High sensitivity and specificity
  • Low limit of detection
  • Rapid, accurate, and cost-effective
  • Easily adapted for use in kits
Applications: 
  • Diagnosis of respiratory disease from sterile clinical samples (e.g., serum, blood, CSF, pleural fluid)
  • Surveillance of pneumococcal carriage from non-sterile samples (e.g., nasopharyngeal swab)
  • Validation studies and proficiency testing
Provider Technology ID: 
3306
Updated On: 
Jul 10, 2018
Date Published: 
Tuesday, July 10, 2018
Provider Classifications: 
Publications: 
Patent Application: 
60/938,799
PCT/US2008/063954
12/600,568
Patent Authority: 
US
PCT
US
Patent Number: 
8,357,488
Licensing Contacts: 
Lead Inventor: 
Inventor IC: 
CDC
CDC
CDC
LPM FIrst Name: 
Karen
LPM Last Name: 
Surabian
LPM Address: 
BG 5601FL RM 2G48 MSC 9804 5601 FISHERS LN
LPM City: 
Bethesda
LPM Zip: 
20892-9804
Inv Is lead: 
LPM State: 
MD
LPM Phone: 
301-594-9719
LPM Suffix: 
J.D., M.B.A.
LPM Organization: 
NIH Office of Technology Transfer
LPM Fax: 
240-627-3117
DTDT Classification: 
Infectious Diseases
Diagnostics
Bacterial
Infectious Diseases (bacterial)
Diagnostic
Monitoring (Public Health/Counterfeit Detection/Biological)
Quality Control/Quality Assurance
Research Tool
Gene-based
In vivo data available (animal)
In vivo data available (human)
DTDT Description: 
Infectious Diseases
Infectious Diseases - Diagnostics
Infectious Diseases - Diagnostics: Bacterial
Infectious Diseases (bacterial)
Diagnostic
Monitoring (Public Health/Counterfeit Detection/Biological)
Quality Control/Quality Assurance
Research Tool
Gene-based
In vivo data available (animal)
In vivo data available (human)
Pat Filing Date: 
2007-05-18
2008-05-16
2010-04-01
Patent Issue Date: 
2013-01-22
Publication Link: 
https://www.ncbi.nlm.nih.gov/pubmed/17537936
https://www.ncbi.nlm.nih.gov/pubmed/23825797
https://www.ncbi.nlm.nih.gov/pubmed/23805203
https://www.ncbi.nlm.nih.gov/pubmed/23224094
https://www.ncbi.nlm.nih.gov/pubmed/23339355
https://www.ncbi.nlm.nih.gov/pubmed/PMID 21471348
Publication Caption: 
PMID 17537936
PMID 23825797
PMID 23805203
[PMID 23224094
PMID 23339355
PMID 21471348
Publication Title: 

Carvalho M., et al.

Carvalho M., et al.

Diaz M., et al.

Pimenta F., et al.

Wu H., et al.

Kodani M., et al.

Technology Term: 
Collaboration Sought: 
Yes
Institute or Center: 
Collaboration Opportunity: 

The CDC Technology Transfer Office (TTO) is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize: Real-time PCR Detection of Streptococcus pneumonia with High Sensitivity and Specificity.
For collaboration opportunities, please contact CDC TTO at tto@cdc.gov or 1-404-639-1330.

E Number Only: 
E-288-2013-0
Inventor First Name: 
Lesley
Maria-Lucia
Maria Da Gloria
Inventor Last Name: 
McGee
Tondella
Carvalho

Original Article: Real-time PCR Detection of Streptococcus pneumoniae with High Sensitivity and Specificity

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