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CRISPR-Based Activation (CRISPRa) of Genes: A How-To Guide

04:00 EDT 4 Oct 2019 | Bitesize Bio

The purpose of this article is to walk you through the steps needed to overexpress genes using CRISPR/Cas9-based activation (CRISPRa). A broader overview of this topic (including CRISPR-based repression) can be found here. CRISPR/Cas9 is more than a programmable nuclease. When stripped of its nuclease activity, it can activate and repress transcription, alter chromatin structure,…

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