Guanine-rich DNA-based peroxidase mimetics for colorimetric assays of alkaline phosphatase.

08:00 EDT 9th October 2015 | BioPortfolio

Summary of "Guanine-rich DNA-based peroxidase mimetics for colorimetric assays of alkaline phosphatase."

DNA-based peroxidase mimetics are facilely constructed through Cu(II)-coordination with different oligonucleotides involving G20, C20, A20 and T20, respectively, with high peroxidase mimicking activity as well as high stability against proteins. Peroxidase-like activities of DNA-Cu(II) complexes are greatly associated with the sequence composition of DNA templates, which decrease in the following order: G20>C20>A20>T20. G20-Cu(II) complex ([Cu(2+)]/[base]=0.05) possesses the Km value of 0.257mM toward 3,3',5,5'-tetramethylbenzidine and 102.3mM toward hydrogen peroxide at 25°C. G20-Cu(II) complexes are employed to develop a colorimetric turn-on assay of alkaline phosphatase with high sensitivity and selectivity, on the basis of pyrophosphate-induced inhibition of their intrinsic peroxidase-like activities. The limit of detection is achieved as 0.84U/L with the linear response region of 20-200U/L. Such colorimetric assay system is probably applicable for the quantitative determination of ALP in biological fluids.


Journal Details

This article was published in the following journal.

Name: Biosensors & bioelectronics
ISSN: 1873-4235
Pages: 549-556


DeepDyve research library

PubMed Articles [18665 Associated PubMed Articles listed on BioPortfolio]

Colorimetric determination of Hg in environmental water based on the Hg-stimulated peroxidase mimetic activity of MoS-Au composites.

A colorimetric assay is described for sensitive determination of Hg ions based on the MoS-Au composites as peroxidase mimetics, which are synthesized by microwave-assisted solvothermal method. The add...

Water dispersed two-dimensional ultrathin Fe(iii)-modified covalent triazine framework nanosheets: peroxidase like activity and colorimetric biosensing applications.

Peroxidase mimics have attracted increasing attention as emerging artificial enzymes due to their promising applications in many fields, including bionanotechnology, sustainable chemistry, and environ...

Peroxidase-Like Activity of Smart Nanomaterials and Their Advanced Application in Colorimetric Glucose Biosensors.

Diabetes is a dominating health issue with 425 million people suffering from the disease worldwide and 4 million deaths each year. To avoid further complications, the diabetic patient blood glucose le...

Intrinsic peroxidase-like activity of CuZnSn(SSe) nanocrystals, and their application to the colorimetric detection of HO.

Nanocrystals (NCs) of type CuZnSn(SSe) (CZTSSe) were prepared via a solvothermal approach. They are shown to be highly efficient peroxidase (POx) mimics for colorimetric detection of HO. By varying th...

Photoactivated oxidase mimetics derived from dicyandiamide and barbituric acid for colorimetric detection of glutathione.

In this work, photoactivated oxidase mimetics was prepared by copolymerizing dicyandiamide with barbituric acid (BA) and characterized by X-ray diffraction pattern, Fourier transformed infrared spectr...

Clinical Trials [3860 Associated Clinical Trials listed on BioPortfolio]

Alkaline Phosphatase Level in Pregnancy and Its Association With Birth Weight

Alkaline phosphatase is known to be produced by syncytiotrophoblasts in the placenta and its levels are normally increased in pregnancy. Therefore, it would be reasonable to hypothesize th...

Search Elevated Serum Alkaline Phosphatase as a Marker of Prenatal Intervillositis Chronic Histiocytic

Background : Chronic histiocytic intervillositis is a rare placental disease linked with poor perinatal outcome. In a recent study, a major antenatal increased alkaline phosphatase was sus...

Effects of Alkaline Phosphatase on Renal Function in Septic Patients

Septic shock is the most common cause of death in patients requiring intensive care. The kidney is one of the first organs to fail, stressing the importance to search for clinical interven...

Alkaline Ionized Water: Sports Recovery and Inflammation Management

The purpose of this study is to determine whether consumption of alkaline ionized water is effective for accelerating sports recovery.

Metabonomics to Investigate Effects of Healthy Diets on Blood Pressure: the OmniHeart Study

To compare the urinary metabolomes on individuals undergone three different healthy diets--a carbohydrate-rich diet, a protein-rich diet, or a diet rich in unsaturated fat.

Medical and Biotech [MESH] Definitions

A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.

Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)

Methods used to measure the relative activity of a specific enzyme or its concentration in solution. Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer.

A nucleic acid sequence that contains an above average number of GUANINE and CYTOSINE bases.

The lectin wheatgerm agglutinin conjugated to the enzyme HORSERADISH PEROXIDASE. It is widely used for tracing neural pathways.

Quick Search


DeepDyve research library

Relevant Topics

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Immunoassay - ELISA
Immunoassays are quick and accurate tests to detect specific molecules. Immunoassays rely on an antibody to bind to the specific structure of a molecule. Antibodies are proteins generated by animals in response to the invasion of a foreign molecule (anti...

An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...

Searches Linking to this Article