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DNA-based peroxidase mimetics are facilely constructed through Cu(II)-coordination with different oligonucleotides involving G20, C20, A20 and T20, respectively, with high peroxidase mimicking activity as well as high stability against proteins. Peroxidase-like activities of DNA-Cu(II) complexes are greatly associated with the sequence composition of DNA templates, which decrease in the following order: G20>C20>A20>T20. G20-Cu(II) complex ([Cu(2+)]/[base]=0.05) possesses the Km value of 0.257mM toward 3,3',5,5'-tetramethylbenzidine and 102.3mM toward hydrogen peroxide at 25°C. G20-Cu(II) complexes are employed to develop a colorimetric turn-on assay of alkaline phosphatase with high sensitivity and selectivity, on the basis of pyrophosphate-induced inhibition of their intrinsic peroxidase-like activities. The limit of detection is achieved as 0.84U/L with the linear response region of 20-200U/L. Such colorimetric assay system is probably applicable for the quantitative determination of ALP in biological fluids.
This article was published in the following journal.
Name: Biosensors & bioelectronics
A colorimetric assay is described for sensitive determination of Hg ions based on the MoS-Au composites as peroxidase mimetics, which are synthesized by microwave-assisted solvothermal method. The add...
Peroxidase mimics have attracted increasing attention as emerging artificial enzymes due to their promising applications in many fields, including bionanotechnology, sustainable chemistry, and environ...
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Alkaline phosphatase is known to be produced by syncytiotrophoblasts in the placenta and its levels are normally increased in pregnancy. Therefore, it would be reasonable to hypothesize th...
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The purpose of this study is to determine whether consumption of alkaline ionized water is effective for accelerating sports recovery.
To compare the urinary metabolomes on individuals undergone three different healthy diets--a carbohydrate-rich diet, a protein-rich diet, or a diet rich in unsaturated fat.
A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.
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A nucleic acid sequence that contains an above average number of GUANINE and CYTOSINE bases.
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Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
Immunoassay - ELISA
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