Temperature Effect on Ionic Current and ssDNA Transport through Nanopores.

08:00 EDT 20th October 2015 | BioPortfolio

Summary of "Temperature Effect on Ionic Current and ssDNA Transport through Nanopores."

We have investigated the role of electrostatic interactions in the transport of nucleic acids and ions through nanopores. The passage of DNA through nanopores has so far been conjectured to involve a free-energy barrier for entry, followed by a downhill translocation where the driving voltage accelerates the polymer. We have tested the validity of this conjecture by using two toxins, α-hemolysin and aerolysin, which differ in their shape, size, and charge. The characteristic timescales in each toxin as a function of temperature show that the entry barrier is ∼15kBT and the translocation barrier is ∼35kBT, although the electrical force in the latter step is much stronger. Resolution of this fact, using a theoretical model, reveals that the attraction between DNA and the charges inside the barrel of the pore is the most dominant factor in determining the translocation speed and not merely the driving electrochemical potential gradient.


Journal Details

This article was published in the following journal.

Name: Biophysical journal
ISSN: 1542-0086
Pages: 1600-1607


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