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Ru(bpy)32+- doped silica (Ru-SiO2) nanoparticles and gold-nanoparticle-decorated graphene (P-RGO@Au) were combined to form P-RGO@Au@Ru-SiO2 composite. And the composite was used to develop a novel sandwich-type electrochemiluminescence immunosensor for the analysis of HIV-1 p24 antigen. The composite worked as carrier to immobilize target antibody and to build a sandwich-type electrochemiluminescence immunosensor through the interaction between antigen and antibody. Importantly, high ECL signal could be obtained due to the large amounts of Ru(bpy)32+ molecules in per Ru-SiO2 nanoparticle. P-RGO@Au composite with good conductivity and high surface area not only accelerated the electron transfer rate, but also improved the loading of both ECL molecules and capture antibody, which could further increase the ECL response and result in high sensitivity. Taking advantage of both Ru-SiO2 nanoparticles and P-RGO@Au composite, the proposed immunosensor exhibited a linear range from 1.0 ×10-9 to 1.0 × 10-5 mg mL-1 with a detection limit of 1.0 ×10-9 mg mL-1 for HIV-1 p24 antigen. The proposed ECL immunosensor was used to analyze HIV-1 p24 antigen in human serum, and satisfactory recoveries were obtained, indicating that the proposed method is promising for practical application in clinical diagnosis of HIV infection.
This article was published in the following journal.
Name: ACS applied materials & interfaces
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A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)
Immunochemical analysis which measures specific target antigen bound to antibody complex. Antibodies in assay form insoluble light scattering immune-aggregates which cause changes in the measured turbidity proportional to the concentration of the antigen.
Philosophy based on the analysis of the individual's existence in the world which holds that human existence cannot be completely described in scientific terms. Existentialism also stresses the freedom and responsibility of the individual as well as the uniqueness of religious and ethical experiences and the analysis of subjective phenomena such as anxiety, guilt, and suffering. (APA, Thesaurus of Psychological Index Terms, 8th ed.)
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Human Immuno Deficiency Virus (HIV)
Human Immunodeficiency Virus (HIV), the causative agent of AIDS. The Human Immunodeficiency Virus, more commonly known as HIV, is a member of the lentivirus sub-set of the retrovirus family of pathogens. It causes AIDS, or Acquired Immuno Deficiency Sy...
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