Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus.

08:00 EDT 21st October 2015 | BioPortfolio

Summary of "Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus."

The crystal structures of the wild-type and catalytic mutant Asp-312→Gly in complex with isomaltohexaose of endo-1,6-dextranase from the thermophilic bacterium Thermoanaerobacter pseudethanolicus (TpDex), belonging to the glycoside hydrolase family 66, were determined. TpDex consists of three structural domains, a catalytic domain comprising an (β/α)8-barrel and two β-domains located at both N- and C-terminal ends. The isomaltohexaose-complex structure demonstrated that the isomaltohexaose molecule was bound across the catalytic site, showing that TpDex had 6 subsites (-4 to +2) in the catalytic cleft. Marked movement of the Trp-376 side-chain along with loop 6, which was the side wall component of the cleft at subsite +1, was observed to occupy subsite +1, indicating that it might expel the cleaved aglycone subsite after the hydrolysis reaction. Structural comparison with other mesophilic enzymes indicated that several structural features of TpDex, loop deletion, salt bridge, and surface-exposed charged residue, may contribute to thermostability.


Journal Details

This article was published in the following journal.

Name: Journal of biochemistry
ISSN: 1756-2651


DeepDyve research library

PubMed Articles [7343 Associated PubMed Articles listed on BioPortfolio]

Creating Machine Learning-Driven Material Recipes Based on Crystal Structure.

Determining the manner in which crystal structures are formed is considered a great mystery within materials science. Potential solutions have the possibility to be uncovered by revealing hidden patte...

Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity.

The biochemical properties of a putative thermostable cycloisomaltooligosaccharide (CI) glucanotransferase gene from Thermoanaerobacter thermocopriae were determined using a recombinant protein (TtCIT...

Structure and function of the putative thioredoxin 1 from the thermophilic eubacterium Thermosipho africanus strain TCF52B.

The thioredoxin system is a ubiquitous oxidoreductase system that consists of the enzyme thioredoxin reductase (TrxR), its cofactor nicotinamide adenine dinucleotide phosphate (NAD(P)H) and the protei...

Crystal structure of the major quadruplex formed in the promoter region of the human c-MYC oncogene.

The c-MYC oncogene mediates multiple tumor cell survival pathways and is dysregulated or overexpressed in the majority of human cancers. The NHE III1 region of the c-MYC promoter forms a DNA quadruple...

The Effect of Crystallinity and Crystal Structure on the Immersion Freezing of Alumina.

Determining the factors that constitute an efficient ice nucleus is an ongoing area of research in the atmospheric community. In particular, surface characteristics such as functional groups and surfa...

Clinical Trials [834 Associated Clinical Trials listed on BioPortfolio]

Co-crystal E-58425 vs Tramadol and Celecoxib for Moderate to Severe Acute Pain After Bunionectomy. Phase III Clinical Trial.

This is a Phase 3, randomized, double-blind, controlled, parallel-group, multicenter clinical trial with co-crystal E-58425 compared to tramadol, to celecoxib, and to placebo. The primary ...

Effectiveness of Liquid Crystal Contact Thermography in Detecting Pathological Changes in Female Breasts

The study is designed to evaluate the effectiveness of liquid crystal contact thermography in detecting pathological changes in female breasts compared to standard diagnostic methods.

Patient Satisfaction and Peri-Implant Tissue Success of Hybrid Ceramic Versus E-Max Superstructures

In patients receiving implants in the esthetic zone, would the use of new Nano hybrid ceramic crown material (Crystal Ultra) improve esthetic patient satisfaction and peri-implant tissue s...

A Pilot Study on Use of Liquid Crystal Contact Thermography to Detect Early Breast Cancer

The study was a multicentre, observational, cross-sectional, open and monitored trial involving 274 females who were subject to an examination using liquid crystal contact thermography dev...

A New Interdisciplinary Collaboration Structure to Improve Medication Safety in the Elderly

Suboptimal use of medications among geriatric patients is well-known problem and leads to medication errors, re-hospitalizations and death. By using a randomized controlled trial (RCT) des...

Medical and Biotech [MESH] Definitions

The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A central nervous system stimulant and sympathomimetic with actions and uses similar to DEXTROAMPHETAMINE. The smokable form is a drug of abuse and is referred to as crank, crystal, crystal meth, ice, and speed.

Enzyme that hydrolyzes DEXTRAN to OLIGOSACCHARIDES.

The use of a quartz crystal microbalance for measuring weights and forces in the micro- to nanogram range. It is used to study the chemical and mechanical properties of thin layers, such as polymer coatings and lipid membranes; and interactions between molecues.

A genus of gram-positive, anaerobic bacteria in the family Thermoanaerobacteriaceae. Cultures consist of rods interspersed with coccoid cells.

Quick Search


DeepDyve research library

Searches Linking to this Article