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Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC).

07:00 EST 2nd February 2017 | BioPortfolio

Summary of "Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC)."

We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which recovers the phase of a sample from images with asymmetric illumination. We employ partially coherent illumination to achieve resolution corresponding to 2× the objective NA. Quantitative phase can then be used to synthesize DIC and phase contrast images or extract shape and density. We demonstrate amplitude and phase recovery at camera-limited frame rates (50 fps) for various in vitro cell samples and c. elegans in a micro-fluidic channel.

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This article was published in the following journal.

Name: PloS one
ISSN: 1932-6203
Pages: e0171228

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