Cytotoxic effects of Fritillaria imperialis L. extracts on human liver cancer cells, breast cancer cells and fibroblast-like cells.

08:00 EDT 4th August 2017 | BioPortfolio

Summary of "Cytotoxic effects of Fritillaria imperialis L. extracts on human liver cancer cells, breast cancer cells and fibroblast-like cells."

In this study, we evaluated the cytotoxic effects of Fritillaria imperialis L. aqueous and ethanolic extracts on human liver cancer cells (LCL-PI 11), breast adenocarcinoma cells (MCF-7) and fibroblast-like cells (HSkMC). MTT, BrdU and TUNEL assays were used to detect cytotoxicity, proliferation rate and apoptotic death. Following 48h, the IC50 values for LCL-PI11 and MCF-7 cells were 4.2 and 3.9μg/mL of aqueous extract, and 1.7 and 1.3μg/mL of ethanolic extract, respectively, which was comparable to that of 5-FU. BrdU assay data verified that both extracts inhibited cell proliferation more preferentially on the two cancer cells. Exposure of LCL-PI 11 and MCF-7 cells to 2.4μg/mL of aqueous extract for 24h resulted in 29% and 32% apoptotic death. Surprisingly the ethanolic extract killed nearly all of the cells at 1.6μg/mL concentration. Collectively, our data indicated that both extracts have cytotoxic, cytostatic and pro-apoptotic activities against the two cancer cell lines. The ethanolic extract was more potent than the aqueous extract and the fibroblast cell was found to be less influenced than both cancer cell lines. Further researches are necessary for chemical characterization and in vivo evaluation especially in animal models to identify the effective anticancer ingredient of F. imperialis.


Journal Details

This article was published in the following journal.

Name: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Pages: 598-604


DeepDyve research library

PubMed Articles [37488 Associated PubMed Articles listed on BioPortfolio]

Qualitative HPLC-DAD-ESI-TOF-MS analysis, cytotoxic and apoptotic effects of Croatian endemic Centaurea ragusina L. aqueous extracts.

Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical c...

Antiproliferative activity of Ontario grown onions against colorectal adenocarcinoma cells.

Cancer is the leading cause of mortality in Canada and other industrialized nations; the development of new/improved cancer therapies is desperately needed and continues to be a major focus of cancer ...

Antioxidant, antimicrobial, antiparasitic, and cytotoxic properties of various Brazilian propolis extracts.

Propolis is known for its biological properties and its preparations have been continuously investigated in an attempt to solve the problem of their standardization, an issue that limits the use of pr...

Cytotoxic Effects of Hydroxylated Fullerenes in Three Types of Liver Cells.

Fullerenes C60 have attracted considerable attention in the biomedical field due to their interesting properties. Although there has been a concern that C60 could be metabolized to hydroxylated fuller...

Three new sesquiterpenes from Pterocarpus santalinus.

Three new sesquiterpenes of canusesnol K (1), canusesnol L (2) and 12, 15-dihydroxycurcumene (3), along with five known ones (4-8), were isolated from the heartwood extract of Pterocarpus santalinus. ...

Clinical Trials [14479 Associated Clinical Trials listed on BioPortfolio]

Effects of Tea Catechin Extracts on Oxidative Damage

The purpose of this study is to clarify the effects of tea catechin extracts on the frequency changes of micronuclei in peripheral lymphocytes compared with other oxidative stress markers ...

The Effect of Vaccinium Myrtillus L. Extract Intake on Human Metabolism

Advanced glycation end-products (AGEs) has been linked to ageing, and many metabolic diseases. The findings of previous experiments suggested that the extracts from polyphenol-rich bilberr...

Comparing Allergenic Effects of 3 German Cockroach Extracts in Adults

The purpose of this study is to test and compare the allergenic effects of three commercially available German cockroach allergen extracts in adults. Study hypothesis: The biological pote...


The study is being conducted in patients in which breast cancer has come back after standard treatment. Volunteers are in this research study are treated using special immune system cells ...

Multi-Tracer PET in Early Phase Trials

The treatment of cancer is increasingly aimed at molecular targets derived from studies of the oncogenes and tumor suppressors known to be involved in the development of human cancers. The...

Medical and Biotech [MESH] Definitions

Extracts of liver tissue containing uncharacterized specific factors with specific activities; a soluble thermostable fraction of mammalian liver is used in the treatment of pernicious anemia.

Tumors or cancer of the LIVER.

One of several basic proteins released from EOSINOPHIL cytoplasmic granules. Eosinophil major basic protein is a 14-kDa cytotoxic peptide with a pI of 10.9. In addition to its direct cytotoxic effects, it stimulates the release of variety of INFLAMMATION MEDIATORS.

A plant genus of the family VERBENACEAE. Lippsidoquinone; TRITERPENES; SESQUITERPENES; and THYMOL have been found in this genus. Plant extracts have cytotoxic activity. It is sometimes called Mexican oregano but that confuses it with real oregano (ORIGANUM).

The transference of a part of or an entire liver from one human or animal to another.

Quick Search

DeepDyve research library

Relevant Topics

Hepatology is the study of liver, gallbladder, biliary tree, and pancreas, and diseases associated with them. This includes viral hepatitis, alcohol damage, cirrhosis and cancer. As modern lifestyles change, with alcoholism and cancer becoming more promi...

  Bladder Cancer Brain Cancer Breast Cancer Cancer Cervical Cancer Colorectal Head & Neck Cancers Hodgkin Lymphoma Leukemia Lung Cancer Melanoma Myeloma Ovarian Cancer Pancreatic Cancer ...

Breast Cancer
Track and monitor developments in breast cancer research and commercial development.  Follow the tabs above to read the latest global news, research, clinical trials on breast cancer and follow companies active in the development of breast cancer tr...

Searches Linking to this Article