Track topics on Twitter Track topics that are important to you
Adult house flies (Musca domestica L.) ingest variable numbers of bacteria when they encounter microbe-rich substrates. Bacterial abundance may affect replication within the fly gut, which subsequently impacts vector potential. This study investigated the dose-dependent replication of GFP-expressing Salmonella enterica serovar Typhimurium (ex Kauffmann and Edwards1952) Le Minor and Popoff 1987, (Enterobacteriales: Enterobacteriaceae) (GFP S. Typhimurium) within the fly alimentary canal. Adult house flies were fed two doses (colony forming units, CFU) of GFP S. Typhimurium (high, ~105 CFU and low, ~104 CFU). Bacteria were examined at 2-, 4-, 6-, 12-, and 24-h postingestion (PI) in situ in the gut via epifluorescence microscopy and enumerated by culture on selective media. In both treatment groups, GFP S. Typhimurium proliferated and persisted in flies for 24 h. In the high-dose group, proliferation peaked at 6 h PI (>500% increase). In the low-dose group, proliferation peaked at both 4 and 6 h PI (>900% increase). Dose significantly affected bacterial replication within the house fly alimentary canal, particularly at 4-, 6-, and 12-h PI. The ability of S. Typhimurium to proliferate and persist in the alimentary canal demonstrates that house flies may serve as significant reservoirs and probable disseminators of this pathogen. Our results show that bacterial abundance should be considered when assessing the potential of house flies to harbor and transmit pathogens.
This article was published in the following journal.
Name: Journal of medical entomology
Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B.
Plasmid-mediated mobilized colistin resistance is currently known to be caused by phosphoethanolamine transferases termed MCR-1, MCR-2, MCR-3 and MCR-4. However, this study focuses on the dissection o...
Successful therapy of a multi-resistant EBSL (SHV-12)-producing and fluoroquinolone-resistant Salmonella enterica subsp. enterica serovar Typhi infection using combination therapy of meropenem and fosfomycin.
We report a traveler who aquired a Salmonella enterica subsp. enterica serovar Typhi strain with resistance against beta-lactams, cephalosporins (ESBL-SHV-12) and quinolones (PMQR qnrB7). After clinic...
Enteric fever remains a major public health problem in low resource settings and antibiotic resistance is increasing. In Asia, an increasing proportion of infections is caused by Salmonella enterica s...
Salmonella Typhimurium (Le Minor and Popoff 1987; Enterobacteriales: Enterobacteriaceae) is a pathogen that causes gastroenteritis in humans and can be harbored by house flies. Factors influencing exc...
We report a typhoid fever case with a Salmonella enterica serovar Typhi isolate showing extended spectrum β-lactamase (ESBL) production in the Democratic Republic of the Congo. Whole genome sequencin...
The purpose of this study is to determine whether CVD 1902 (a live, attenuated, oral vaccine) is safe and effective in the prevention of Salmonella enterica serovar paratyphi A infection.
In this Phase I clinical study, three recombinant, avirulent Salmonella Typhi (RASV) strains each expressing the Streptococcus pneumoniae surface protein, PspA, will be compared as live bi...
To determine the clinical significance of gene mutations encoding antibiotic resistance, but not expressed as clinical resistance, in Salmonella bacteria
HIV-1 cannot be eradicated from infected individuals by current antiretroviral regimens. Cellular reservoirs and tissue sanctuary sites allow latent HIV-1 persistence and ongoing low-level...
The working hypothesis is that oral administration of an attenuated strain of Salmonella typhimurium is safe and efficacious for patients with unresectable hepatic metastasis from a solid ...
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
A serotype of Salmonella enterica which is an etiologic agent of gastroenteritis in man and other animals.
A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER.
A serotype of SALMONELLA ENTERICA which is an agent of PARATYPHOID FEVER in humans.
A serotype of SALMONELLA ENTERICA that causes mild PARATYPHOID FEVER in humans.
Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...