Dose-Dependent Effects on Replication and Persistence of Salmonella enterica serovar Typhimurium in House Flies (Diptera: Muscidae).

08:00 EDT 4th October 2017 | BioPortfolio

Summary of "Dose-Dependent Effects on Replication and Persistence of Salmonella enterica serovar Typhimurium in House Flies (Diptera: Muscidae)."

Adult house flies (Musca domestica L.) ingest variable numbers of bacteria when they encounter microbe-rich substrates. Bacterial abundance may affect replication within the fly gut, which subsequently impacts vector potential. This study investigated the dose-dependent replication of GFP-expressing Salmonella enterica serovar Typhimurium (ex Kauffmann and Edwards1952) Le Minor and Popoff 1987, (Enterobacteriales: Enterobacteriaceae) (GFP S. Typhimurium) within the fly alimentary canal. Adult house flies were fed two doses (colony forming units, CFU) of GFP S. Typhimurium (high, ~105 CFU and low, ~104 CFU). Bacteria were examined at 2-, 4-, 6-, 12-, and 24-h postingestion (PI) in situ in the gut via epifluorescence microscopy and enumerated by culture on selective media. In both treatment groups, GFP S. Typhimurium proliferated and persisted in flies for 24 h. In the high-dose group, proliferation peaked at 6 h PI (>500% increase). In the low-dose group, proliferation peaked at both 4 and 6 h PI (>900% increase). Dose significantly affected bacterial replication within the house fly alimentary canal, particularly at 4-, 6-, and 12-h PI. The ability of S. Typhimurium to proliferate and persist in the alimentary canal demonstrates that house flies may serve as significant reservoirs and probable disseminators of this pathogen. Our results show that bacterial abundance should be considered when assessing the potential of house flies to harbor and transmit pathogens.


Journal Details

This article was published in the following journal.

Name: Journal of medical entomology
ISSN: 1938-2928


DeepDyve research library

PubMed Articles [22080 Associated PubMed Articles listed on BioPortfolio]

Three Siphoviridae Salmonella enterica serovar Enteritidis bacteriophage are promising candidates for phage therapy.

Salmonella is a common and widely distributed foodborne pathogen that is frequently implicated in gastrointestinal infections. The emergence and spread of Salmonella strains resistant to multiple anti...

Characterization of stm3030 and stm3031 genes of Salmonella enterica serovar Typhimurium in relation to cephalosporin resistance.

The outer membrane protein STM3031 had been shown to confer Salmonella enterica serovar Typhimurium resistance to ceftriaxone. In this study, the STM3030 was increased in strain R200 and decreased in ...

Type I interferons differentially modulate maternal host immunity to infection by Listeria monocytogenes and Salmonella enterica serovar Typhimurium during pregnancy.

IFN-alpha receptor deficiency (IFNAR ) enhances immunity to Listeria monocytogenes (LM) and Salmonella enterica serovar Typhimurium (ST) in the non-pregnant state by inhibiting pathogen-induced immune...

An IncX1 plasmid isolated from Salmonella enterica subsp. enterica serovar Pullorum carrying bla, sul2, arsenic resistant operons.

We have identified an IncX1 plasmid named pQJDSal1 from Salmonella enterica subsp. enterica serovar Pullorum (S. Pullorum). The plasmid is 67,685 bp in size and has 72 putative genes. pQJDSal1 harbo...

Enhanced survival following oral and systemic Salmonella enterica serovar Typhimurium infection in polymeric immunoglobulin receptor knockout mice.

Polymeric immunoglobulin receptor (pIgR) transport of secretory immunoglobulin A (SIgA) to mucosal surfaces is thought to promote gut integrity and immunity to Salmonella enterica serovar Typhimurium ...

Clinical Trials [9481 Associated Clinical Trials listed on BioPortfolio]

Phase I Safety and Efficacy Study of CVD 1902, a Live, Attenuated Oral Vaccine to Prevent Salmonella Enterica Serovar Paratyphi A Infection

The purpose of this study is to determine whether CVD 1902 (a live, attenuated, oral vaccine) is safe and effective in the prevention of Salmonella enterica serovar paratyphi A infection.

Recombinant Attenuated Salmonella Typhi Vaccine Vectors Producing Streptococcus Pneumoniae PspA

In this Phase I clinical study, three recombinant, avirulent Salmonella Typhi (RASV) strains each expressing the Streptococcus pneumoniae surface protein, PspA, will be compared as live bi...

NCCLS Interpretive Criteria for Salmonella

To determine the clinical significance of gene mutations encoding antibiotic resistance, but not expressed as clinical resistance, in Salmonella bacteria

Residual Replication of HIV-1 in the Gut Associated Lymphoid Tissue (GALT) of Patients on Highly Active Antiretroviral Therapy (HAART): the ANRS EP 44 Study

HIV-1 cannot be eradicated from infected individuals by current antiretroviral regimens. Cellular reservoirs and tissue sanctuary sites allow latent HIV-1 persistence and ongoing low-level...

IL-2 Expressing, Attenuated Salmonella Typhimurium in Unresectable Hepatic Spread

The working hypothesis is that oral administration of an attenuated strain of Salmonella typhimurium is safe and efficacious for patients with unresectable hepatic metastasis from a solid ...

Medical and Biotech [MESH] Definitions

A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.

A serotype of SALMONELLA ENTERICA that causes mild PARATYPHOID FEVER in humans.

A serotype of Salmonella enterica which is an etiologic agent of gastroenteritis in man and other animals.

A serotype of SALMONELLA ENTERICA which is an agent of PARATYPHOID FEVER in humans.

A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER.

Quick Search


DeepDyve research library

Relevant Topic

Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...

Searches Linking to this Article