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Cell Surface Display of MerR on Saccharomyces cerevisiae for Biosorption of Mercury.

07:00 EST 11th November 2017 | BioPortfolio

Summary of "Cell Surface Display of MerR on Saccharomyces cerevisiae for Biosorption of Mercury."

The metalloregulatory protein MerR which plays important roles in mer operon system exhibits high affinity and selectivity toward mercury (II) (Hg(2+)). In order to improve the adsorption ability of Saccharomyces cerevisiae for Hg(2+), MerR was displayed on the surface of S. cerevisiae for the first time with an α-agglutinin-based display system in this study. The merR gene was synthesized after being optimized and added restriction endonuclease sites EcoR I and Mlu I. The display of MerR was indirectly confirmed by the enhanced adsorption ability of S. cerevisiae for Hg(2+) and colony PCR. The hydride generation atomic absorption spectrometry was applied to measure the Hg(2+) content in water. The engineered yeast strain not only showed higher tolerance to Hg, but also their adsorption ability was much higher than that of origin and control strains. The engineered yeast could adsorb Hg(2+) under a wide range of pH levels, and it could also adsorb Hg(2+) effectively with Cd(2+) and Cu(2+) coexistence. Furthermore, the engineered yeast strain could adsorb ultra-trace Hg(2+) effectively. The results above showed that the surface-engineered yeast strain could adsorb Hg(2+) under complex environmental conditions and could be used for the biosorption and bioremediation of environmental Hg contaminants.

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This article was published in the following journal.

Name: Molecular biotechnology
ISSN: 1559-0305
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Medical and Biotech [MESH] Definitions

A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS from SACCHAROMYCES CEREVISIAE. It is involved in morphological events related to the cell cycle. This enzyme was formerly listed as EC 3.6.1.47.

Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.

A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.

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