Nuclear myosin 1 associates with papillomavirus E2 regulatory protein and influences viral replication.

07:00 EST 24th November 2017 | BioPortfolio

Summary of "Nuclear myosin 1 associates with papillomavirus E2 regulatory protein and influences viral replication."

Nuclear myosin 1c (NM1) associates with RNA polymerases and is a partner in the chromatin remodeling complex B-WICH. This complex, which also contains WSTF and SNF2h proteins, is involved in transcriptional regulation. We report herein that papillomavirus protein E2 binds to NM1 and co-precipitates with the WSTF and SNF2h proteins. Our data suggest that E2 associates with the cellular B-WICH complex through binding to NM1. E2 and NM1 associate via their N-terminal domains and this interaction is ATP dependent. The cellular multifunctional protein Brd4 and beta-actin are also present in the NM1-E2 complex. NM1 downregulation by siRNA increases the replication of the BPV1 and HPV5 genomes but does not affect HPV18 genome replication. These results suggest that the B-WICH complex may play a role in the papillomavirus life cycle through NM1 and E2 protein interaction.


Journal Details

This article was published in the following journal.

Name: Virology
ISSN: 1096-0341
Pages: 142-155


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