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Ultra-high performance liquid chromatography tandem mass spectrometry for cyclosporine analysis in human whole blood and comparison with an antibody-conjugated magnetic immunoassay.

07:00 EST 4th December 2017 | BioPortfolio

Summary of "Ultra-high performance liquid chromatography tandem mass spectrometry for cyclosporine analysis in human whole blood and comparison with an antibody-conjugated magnetic immunoassay."

Various immunoassays have been used for cyclosporine A (CsA) analysis in human whole blood; however, they could not fully satisfy the requirements of criteria for accuracy and specificity in CsA measurement. The liquid chromatography tandem mass spectrometry is a gold method for CsA analysis. The aim of the study was to develop and validate an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for CsA analysis and establish its agreement with an antibody-conjugated magnetic immunoassay (ACMIA) in clinical sample analysis.

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This article was published in the following journal.

Name: Therapeutic drug monitoring
ISSN: 1536-3694
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Medical and Biotech [MESH] Definitions

A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.

A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.

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