Glutathione levels influence chronological life span of Saccharomyces cerevisiae in a glucose-dependent manner.

07:00 EST 26th December 2017 | BioPortfolio

Summary of "Glutathione levels influence chronological life span of Saccharomyces cerevisiae in a glucose-dependent manner."

Diet plays a key role in determining the longevity of the organisms since it has been demonstrated that glucose restriction increases lifespan whereas a high-glucose diet decreases it. However, the molecular basis of how diet leads to the aging process is currently unknown. We propose that the quantity of glucose that fuels respiration influences ROS generation and glutathione levels, and both chemical species impact in the aging process. Herein, we provide evidence that mutation of the gene GSH1 in S. cerevisiae diminishes glutathione levels. Moreover, glutathione levels were higher with 0.5% than in 10% glucose in the gsh1Δ and WT strains. Interestingly, the chronological life span (CLS) was lowered in the gsh1Δ strain cultured with 10% glucose but not under dietary restriction. The gsh1Δ strain also showed an inhibition of the mitochondrial respiration in 0.5 and 10% of glucose but only increased the H2 O2 levels under dietary restriction. These results correlate well with the GSH/GSSG ratio, which showed a decrease in gsh1Δ strain cultured with 0.5% glucose. Together, these data indicate that glutathione exhaustion impact negatively both the electron transport chain function and the CLS of yeast, the latter occurring when a low threshold level of this antioxidant is reached, independently of the H2 O2 levels.


Journal Details

This article was published in the following journal.

Name: Yeast (Chichester, England)
ISSN: 1097-0061


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