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A rare variant of walnut with a red seed coat (pellicle) was examined for alterations in the phenolic profiles during development. The red walnut (RW) pellicle was compared to two commonly-colored walnut varieties; 'Lara' (brown) and 'Fernor' (light brown). Furthermore, the activity of selected enzymes of the phenylpropanoid and flavonoid related pathways and the relative expression of phenylalanine ammonia lyase (PAL) and anthocyanidin synthase (ANS) structural genes were examined in the pellicles of the three varieties. In the pellicle of RW, phenylalanine ammonia lyase (PAL) activity and the related PAL expression was most pronounced in August, about one month before commercial maturity, suggesting a high synthesis rate of phenolic compounds at this development stage. The most pronounced differences between the red and the light/dark brown varieties were the increased activity/gene expression of PAL and the increased anthocyanidin synthase (ANS) expression of RW in August. The vibrant color of the RW pellicle is based on the presence of four derivatives of cyanidin and delphinidin-hexosides.
This article was published in the following journal.
Name: Journal of agricultural and food chemistry
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SUPERVISED MACHINE LEARNING algorithm which learns to assign labels to objects from a set of training examples. Examples are learning to recognize fraudulent credit card activity by examining hundreds or thousands of fraudulent and non-fraudulent credit card activity, or learning to make disease diagnosis or prognosis based on automatic classification of microarray gene expression profiles drawn from hundreds or thousands of samples.
The use of techniques that produce a functional MUTATION or an effect on GENE EXPRESSION of a specific gene of interest in order to identify the role or activity of the gene product of that gene.
The expression of a gene in an abnormal place, or at an abnormal time in an organism. Ectopic Gene Expression is often induced artificially by genetic techniques.
A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.
Techniques used to add in exogenous gene sequence such as mutated genes; REPORTER GENES, to study mechanisms of gene expression; or regulatory control sequences, to study effects of temporal changes to GENE EXPRESSION.
The process of gene expression is used by eukaryotes, prokaryotes, and viruses to generate the macromolecular machinery for life. Steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-tran...