Dose-dependent quantitative effects of acute fructose administration on hepatic de novo lipogenesis in healthy humans.

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Summary of "Dose-dependent quantitative effects of acute fructose administration on hepatic de novo lipogenesis in healthy humans."

Fructose feeding increases hepatic de novo lipogenesis (DNL) and is associated with non-alcoholic fatty liver disease. Little is known, however, about individual variation in susceptibility to fructose stimulation of DNL. In this three-period, cross-over study, seventeen healthy male subjects were enrolled to evaluate the within and between subject variability of acute fructose feeding on hepatic fractional DNL. During each assessment, [1-C]-acetate was infused to measure DNL in the fasting state and during fructose feeding. Subjects randomly received a high-dose of fructose (10 mg/kg fat free mass/min) on two occasions and a low-dose (5 mg/kg fat free mass/min) on another. Fructose solutions were administered orally every 30 min for 9.5 hours. Ten subjects completed all three study periods. DNL was assessed as the fractional contribution of newly synthesized palmitate into very-low-density-lipoprotein triglycerides using mass isotopomer distribution analysis. Mean fasting DNL was 5.3%{plus minus} 2.8% with significant within and between subject variability. DNL increased dose-dependently during fructose feeding to 15{plus minus}2% for low- and 29{plus minus}2% for high-dose fructose. The DNL response to high-dose fructose was very reproducible within an individual (r=0.93, P<0.001) and independent of fasting DNL. However, it was variable between individuals and significantly correlated to influx of unlabeled acetyl-CoA (r=0.7, P<0.001). Unlike fasting DNL, fructose stimulated DNL is a robust and reproducible measure of hepatic lipogenic activity for a given individual and may be a useful indicator of metabolic disease susceptibility and treatment response.


Journal Details

This article was published in the following journal.

Name: American journal of physiology. Endocrinology and metabolism
ISSN: 1522-1555


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