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Genome-wide identification of lectin receptor kinases in pear: Functional characterization of the L-type LecRLK gene PbLRK138.

08:00 EDT 27th March 2018 | BioPortfolio

Summary of "Genome-wide identification of lectin receptor kinases in pear: Functional characterization of the L-type LecRLK gene PbLRK138."

Lectin receptor-like kinases (LecRLKs) are membrane-bound receptors that are believed to be involved in biotic and abiotic stress responses. However, little is known about the LecRLK family in pear. In this study, a total of 172 LecRLK genes were first identified in the entire pear genome. The 172 LecRLKs were divided into three types (111 G-, 59 L- and two C-types) based on their structure and phylogenetic relationships. LecRLK gene expressions were detected in different pear tissues including roots, stems, leaves, flowers and fruits, and the most of the 11 selected LecRLKs exhibited similar expression patterns. Furthermore, six selected LecRLKs were shown to be involved in salt stress response. Overexpression of PbLRK138, an L-type LecRLK, caused cell death and induced expression of defense-related genes in Nicotiana benthamiana. Two deletion mutants containing lectin or transmembrane and serine/threonine kinase domains did not trigger cell death. In addition, only the mutant with the transmembrane domain was localized to the plasma membrane.

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Name: Gene
ISSN: 1879-0038
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Medical and Biotech [MESH] Definitions

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