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Secretory overexpression of Bacillus thermocatenulatus lipase in Saccharomyces cerevisiae using combinatorial library strategy.

08:00 EDT 10th April 2018 | BioPortfolio

Summary of "Secretory overexpression of Bacillus thermocatenulatus lipase in Saccharomyces cerevisiae using combinatorial library strategy."

Simple and cost-effective lipase expression host microorganisms are highly desirable. A combinatorial library strategy was used to improve the secretory expression of lipase from Bacillus thermocatenulatus (BTL2) in the culture supernatant of Saccharomyces cerevisiae. A plasmid library including expression cassettes composed of sequences encoding one of each 15 promoters, 15 secretion signals, and 15 terminators derived from yeast species, S. cerevisiae, Pichia pastoris, and Hansenula polymorpha, was constructed. The S. cerevisiae transformant YPH499/D4, comprising H. polymorpha GAP promoter, S. cerevisiae SAG1 secretion signal, and P. pastoris AOX1 terminator, was selected by high-throughput screening. This transformant expressed BTL2 extra-cellularly with a 130-fold higher than the control strain, comprising S. cerevisiae PGK1 promoter, S. cerevisiae α-factor secretion signal, and S. cerevisiae PGK1 terminator, after cultivation for 72 h. This combinatorial library strategy holds promising potential for application in the optimization of the secretory expression of proteins in yeast.

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This article was published in the following journal.

Name: Biotechnology journal
ISSN: 1860-7314
Pages: e1700409

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