Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing.

08:00 EDT 8th June 2018 | BioPortfolio

Summary of "Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing."

Malaria parasites are transmitted through blood feeding by female Anopheline mosquitoes. Unveiling the blood-feeding process will improve understanding of vector biology. Anopheles dirus (Diptera: Culicidae) is one of the primary malaria vectors in the Greater Mekong Subregion, the epicentre of malaria drug resistance. In this study, differential gene expression between sugar- and blood-fed An. dirus was investigated by RNA sequencing (RNA-seq). A total of 589 transcripts were found to be upregulated and 703 transcripts downregulated as a result of blood feeding. Transcriptional differences were found in genes involved in blood digestion, peritrophic matrix formation, oogenesis and vitellogenesis. The expression levels of several genes were validated by quantitative reverse transcription polymerase chain reaction. The present results provide better understanding of An. dirus biology in relation to its blood feeding.


Journal Details

This article was published in the following journal.

Name: Medical and veterinary entomology
ISSN: 1365-2915


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Medical and Biotech [MESH] Definitions

The expression of a gene in an abnormal place, or at an abnormal time in an organism. Ectopic Gene Expression is often induced artificially by genetic techniques.

Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.

Methods of studying differential GENE EXPRESSION of especially low-abundance, tissue-specific gene transcripts.

Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.

Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in archaea.

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