Advertisement

Topics

Comparison of multivariate data analysis techniques to improve glucose concentration prediction in mammalian cell cultivations by Raman spectroscopy.

08:00 EDT 7th June 2018 | BioPortfolio

Summary of "Comparison of multivariate data analysis techniques to improve glucose concentration prediction in mammalian cell cultivations by Raman spectroscopy."

In-situ Raman spectroscopy is frequently applied to monitor and even control the glucose concentration of monoclonal antibody producing mammalian cell cultivations. Previous studies used the PLSR algorithm only, however other multivariate algorithms were applied successfully for different protein production processes. In this study, four mammalian cell cultivation runs were followed with Raman spectroscopy and the spectra were analysed quantitatively and qualitatively as well. The PCA analysis showed that one of the most dominant factors in the Raman spectra were the concentration of glucose, which strongly correlated with the score values of the eighth principal component. This observation further substantiated that Raman spectroscopy is an excellent tool for bioprocess monitoring and induced the test of the Multivariate Linear Regression (MLR), Principal Component Regression (PCR) and Partial Least Squares Regression (PLSR) algorithms, using the results of the PCA as one of the variable selection techniques, to determine the glucose concentration during cultivation. However, the novel variable selection technique of PCA correlation enhanced only the model accuracy when it was applied with MLR and only model robustness was increased when it was used with PCR and PLSR because the relatively strong Raman signal of glucose concentration seemed to be enough to build an accurate model on. Therefore, PLSR, the most advanced algorithm of the three, delivered the lowest 2.21 mM RMSEP but it was demonstrated that in certain cases PCR could also produce satisfactorily results.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Pages: 269-279

Links

DeepDyve research library

PubMed Articles [41149 Associated PubMed Articles listed on BioPortfolio]

Testing Mean Differences among Groups: Multivariate and Repeated Measures Analysis with Minimal Assumptions.

To date, there is a lack of satisfactory inferential techniques for the analysis of multivariate data in factorial designs, when only minimal assumptions on the data can be made. Presently available m...

Evaluation of the microscopic distribution of florfenicol in feed pellets for salmon by Fourier Transform infrared imaging and multivariate analysis.

Fourier Transform infrared imaging and multivariate analysis were used to identify, at the microscopic level, the presence of florfenicol (FF), a heavily-used antibiotic in the salmon industry, suppli...

TOWARD IMPROVED ANALYSIS OF CONCENTRATION DATA: EMBRACING NONDETECTS.

Various statistical tests on concentration data serve to support decision-making regarding characterization and monitoring of contaminated media, assessing exposure to a chemical, and quantifying the ...

Calibration of Minimally Invasive Continuous Glucose Monitoring Sensors: State-of-The-Art and Current Perspectives.

Minimally invasive continuous glucose monitoring (CGM) sensors are wearable medical devices that provide real-time measurement of subcutaneous glucose concentration. This can be of great help in the d...

Wearable, robust, non-enzymatic continuous glucose monitoring system and its in vivo investigation.

Electroplating of nanoporous Pt (nPt) induces an extremely strong tensile stress, which results in the exfoliation of nPt on flexible polymer substrate despite plasma treatment to improve adhesion. He...

Clinical Trials [13431 Associated Clinical Trials listed on BioPortfolio]

Wine Consumption and Glycemic Control

Type 2 diabetes is a disease in which the blood glucose concentration remains above normal, which puts one at risk of heart disease, stroke, and kidney and nerve damage. Insulin, which is ...

Comparison of Aqueous and Plasma Glucose Level in Diabetic Patients

Comparison of aqueous and plasma glucose level in diabetic patients High glucose concentrations in aqueous humor are thought to be related of diabetic ocular injury, including cataract an...

Comparison of the Effects of Intermittent and Continuous Enteral Feeding on Glucose-Insulin Dynamics in Critically Ill Medical Patients

Continuous enteral feeding is the most common type of nutrition used in critically ill patients despite being non-physiologic, as all mammalian alimentary tracts have been designed for int...

Interactive Patient's Assistant - LUCY

Patients with chronic heart failure (CHF) and reduced left ventricle ejection fraction benefit from cardiac resynchronization therapy (CRT) and implantable cardioverter defibrillator (ICD)...

Glucose Clamp Study to Prove Hypo- and Hyperglycemic Episodes Using a Non-invasive Glucose Monitoring Device

Continuous monitoring of the skin tissue glucose concentration on two different study days using glucose sensors that work on the principle of impedance spectroscopy, with systematic alter...

Medical and Biotech [MESH] Definitions

A set of techniques used when variation in several variables has to be studied simultaneously. In statistics, multivariate analysis is interpreted as any analytic method that allows simultaneous study of two or more dependent variables.

Maintenance of a constant blood glucose level by perfusion or infusion with glucose or insulin. It is used for the study of metabolic rates (e.g., in glucose, lipid, amino acid metabolism) at constant glucose concentration.

MOLECULAR BIOLOGY techniques used in the diagnosis of disease. Included are such techniques as IN SITU HYBRIDIZATION of chromosomes for CYTOGENETIC ANALYSIS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS of gene expression patterns in disease states; identification of pathogenic organisms by analysis of species specific DNA sequences; and detection of mutations with POLYMERASE CHAIN REACTION.

Systematic gathering of data for a particular purpose from various sources, including questionnaires, interviews, observation, existing records, and electronic devices. The process is usually preliminary to statistical analysis of the data.

Pathological conditions in which the BLOOD GLUCOSE cannot be maintained within the normal range, such as in HYPOGLYCEMIA and HYPERGLYCEMIA. Etiology of these disorders varies. Plasma glucose concentration is critical to survival for it is the predominant fuel for the CENTRAL NERVOUS SYSTEM.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Antibodies
An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens. Examples of antigens include microorganisms (such as bacteria, fungi, parasites, and viruses) and chemicals. Antibodies may be produc...


Searches Linking to this Article