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Pro-Glu/Pro-Pro-Glu (PE/PPE) family proteins in Mycobacterium tuberculosis (Mtb) are contributors to pathogenesis and immune evasion. These proteins have a unique structure in which the sequence is conserved. We investigated the vaccine potential of ESAT-6 fused with the consensus CD4 T-cell epitopes of PE/PPE proteins against a highly pathogenic Mtb strain HN878 in a murine model.We selected the consensus CD4 T-cell epitopes of PE/PPE proteins by multiple alignments and by investigating their IFN-γ response during Mtb infection, and produced their fused ESAT-6 vaccine antigens. Our results showed that an increased immune response was observed in the PE/PPE peptide + ESAT-6 protein immunization group compared with that in the ESAT-6 only immunization group. After challenging with Mtb, we observed induced CD4 T-cells secreting double-positive cytokine IL-2/IFN-γ, which is associated with protective T-cell immunity. Also, lower numbers of colony-forming units were observed in the spleen of the fusion protein immunization groups than in the single ESAT-6 group. Thus, conjugation of the consensus CD4 T-cell epitopes in N terminus of PE/PPE to vaccine antigens could potentially increase the protective efficacy of as a subunit vaccine.
This article was published in the following journal.
Name: Biochemical and biophysical research communications
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A live vaccine containing attenuated poliovirus, types I, II, and III, grown in monkey kidney cell tissue culture, used for routine immunization of children against polio. This vaccine induces long-lasting intestinal and humoral immunity. Killed vaccine induces only humoral immunity. Oral poliovirus vaccine should not be administered to immunocompromised individuals or their household contacts. (Dorland, 28th ed)
A suspension of killed Bordetella pertussis organisms, used for immunization against pertussis (WHOOPING COUGH). It is generally used in a mixture with diphtheria and tetanus toxoids (DTP). There is an acellular pertussis vaccine prepared from the purified antigenic components of Bordetella pertussis, which causes fewer adverse reactions than whole-cell vaccine and, like the whole-cell vaccine, is generally used in a mixture with diphtheria and tetanus toxoids. (From Dorland, 28th ed)
A vaccine consisting of DIPHTHERIA TOXOID; TETANUS TOXOID; and whole-cell PERTUSSIS VACCINE. The vaccine protects against diphtheria, tetanus, and whooping cough.
A combined vaccine used to prevent infection with diphtheria and tetanus toxoid. This is used in place of DTP vaccine (DIPHTHERIA-TETANUS-PERTUSSIS VACCINE) when PERTUSSIS VACCINE is contraindicated.
A live attenuated virus vaccine of duck embryo or human diploid cell tissue culture origin, used for routine immunization of children and for immunization of nonpregnant adolescent and adult females of childbearing age who are unimmunized and do not have serum antibodies to rubella. Children are usually immunized with measles-mumps-rubella combination vaccine. (Dorland, 28th ed)
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