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Sequence requirements of intrinsically fluorescent G-quadruplexes.

08:00 EDT 13th June 2018 | BioPortfolio

Summary of "Sequence requirements of intrinsically fluorescent G-quadruplexes."

G-quadruplexes are four-stranded nucleic acid structures typically stabilized by GGGG tetrads. These structures are intrinsically fluorescent, which expands the known scope of nucleic acid function and raises the possibility that they could eventually be used as signaling components in label-free sensors constructed from DNA or RNA. In this study we systematically investigated the effects of mutations in tetrads, loops, and overhanging nucleotides on the fluorescence intensity and emission wavelength of more than 500 sequence variants of a reference DNA G-quadruplex. Some of these mutations modestly increased the fluorescence intensity of this G-quadruplex, while others shifted its emission wavelength. Mutations that increased fluorescence intensity were distinct from those that increased emission wavelength, suggesting a tradeoff between these two biochemical properties. Fluorescence intensity and emission wavelength were also correlated with multimeric state: the most fluorescent G-quadruplexes were monomers, while those with the highest emission wavelengths typically formed dimeric structures. Oligonucleotides containing multiple G-quadruplexes were in some cases more fluorescent than those containing a single G-quadruplex, although this depended on both the length and sequence of the spacer linking the G-quadruplexes. These experiments provide new insights into the properties of fluorescent G-quadruplexes, and should aid in the development of improved label-free nucleic acid sensors.

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This article was published in the following journal.

Name: Biochemistry
ISSN: 1520-4995
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