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Effects of AttM lactonase on the pathogenicity of Streptomyces scabies.

08:00 EDT 13th June 2018 | BioPortfolio

Summary of "Effects of AttM lactonase on the pathogenicity of Streptomyces scabies."

The biosynthesis of phytotoxin thaxtoimn A (TXT) constitutes the major pathogenicity determinant in Streptomyces scabies, the most widely-studied phytopathogen causing scab disease in potato and other root crops. It is recognized that S. scabies regulates its pathogenicity via γ-butyrolactone (GBL)-dependent quorum sensing (QS) signaling. AttM, from Agrobacterium tumefaciens C58 strain, has recently been proposed to have GBL-assimilative capacity. Here, we presented the introduction of A. tumefaciens-derived attM gene into S. scabies using the E. coli-Streptomyces shuttle vector pIJ8600 via intergeneric conjugation, followed by the investigation of secondary metabolism (mycelium growth, TXT production and pathogenicity) in S. scabies attM exconjugants (S.s/attM) in comparison to their wild-type parent strain (S.s/WT). Among the resultant S.s/attM exconjugants, attM was found to be integrated into S. scabies chromosome as analyzed by southern blotting. Moreover, S.s/attM failed to evoke the disease symptoms in planta and displayed altered morphological differentiation in contrast to S.s/WT. The abolishment of TXT production in S.s/attM substantiated the loss of pathogenicity, and also implied that attM, when constitutively expressed in S. scabies, could paralyze its GBL signaling pathway. Altogether, lactonase-coding gene attM would be useful in a quorum quenching strategy for plant protection via suppressing TXT production and pathogenicity of S. scabies. This article is protected by copyright. All rights reserved.

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Name: Letters in applied microbiology
ISSN: 1472-765X
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