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MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction.

07:00 EST 1st January 2018 | BioPortfolio

Summary of "MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction."

The analysis of microRNA expression patterns provides new insights into numerous cellular processes and their aberrances in diseases. Despite its potential pitfalls, the quantitative real-time polymerase chain reaction (qPCR) is the most commonly used tool for microRNA profiling. The method requires extraction and quality analysis of RNA, which is further reverse transcribed using specific primers and used as a template in a qPCR reaction. All these elements have been addressed in this chapter.

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This article was published in the following journal.

Name: Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Pages: 69-85

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