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The seagrass Zostera marina L. shows optimal growth in marine water and reduced growth under low salinity conditions. However, little is known about the molecular mechanisms underlying its adaptation to high salinity in Z. marina. In this study, transcriptomic analyses were performed using RNA-seq of the following two groups with different NaCl content: the CK group (seagrasses grown in the absence of NaCl) and the NaCl group (seagrasses grown in the presence of 400 mM NaCl for 6 h). Approximately 316 million high-quality reads were generated, and 87.9% of the data were mapped to the reference genome. Moreover, differentially expressed genes between the CK and NaCl groups were identified. According to a functional analysis, the up-regulated genes after the NaCl treatment were significantly enriched in nitrogen metabolism, calcium signalling and DNA replication while the down-regulated genes were significantly enriched in photosynthesis. A comparative transcriptomic analysis detected many differentially expressed genes and pathways required for adaptation to NaCl stress, providing a foundation for future studies investigating the molecular mechanisms of salt adaptation in Z. marina. We discuss how molecular changes in these processes may have contributed to the NaCl adaptation.
This article was published in the following journal.
Name: Plant physiology and biochemistry : PPB
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evaluate efficacy and safety of IV administration of potassium chloride (KCl) plus 0.9%NaCl compare with KCl plus half-strength normal saline solution (0.45%NaCl) in correcting hypokalemia
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The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.
MOLECULAR BIOLOGY techniques used in the diagnosis of disease. Included are such techniques as IN SITU HYBRIDIZATION of chromosomes for CYTOGENETIC ANALYSIS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS of gene expression patterns in disease states; identification of pathogenic organisms by analysis of species specific DNA sequences; and detection of mutations with POLYMERASE CHAIN REACTION.
The description and measurement of the various factors that produce physical stress upon dental restorations, prostheses, or appliances, materials associated with them, or the natural oral structures.
The adjustment of the eye to variations in the intensity of light. Light adaptation is the adjustment of the eye when the light threshold is increased; DARK ADAPTATION when the light is greatly reduced. (From Cline et al., Dictionary of Visual Science, 4th ed)
The sum of all nonspecific systemic reactions of the body to long-continued exposure to systemic stress.
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Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
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