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Evaluation of the immune response against Trypanosoma cruzi cytosolic Tryparedoxin Peroxidase in human natural infection.

08:00 EDT 4th July 2018 | BioPortfolio

Summary of "Evaluation of the immune response against Trypanosoma cruzi cytosolic Tryparedoxin Peroxidase in human natural infection."

Trypanosoma cruzi, the etiological agent of Chagas disease, has a highly efficient detoxification system to deal with the oxidative burst imposed by its host. One of the antioxidant enzymes involved is the cytosolic tryparedoxin peroxidase (c-TXNPx) which catalyzes the reduction of hydrogen peroxide, small-chain organic hydroperoxides and peroxynitrite. This enzyme is present in all parasite stages, and its over-expression renders parasites more resistant to oxidative defenses of macrophages, favoring parasite survival. This work addressed the study of the specific humoral and cellular immune response triggered by c-TXNPx in human natural infection. Thus, sera and peripheral blood mononuclear cells (PBMC) were collected from chronic asymptomatic and cardiac patients, and non-infected individuals. Results showed that levels of IgG antibodies against c-TXNPx were low in sera from individuals across all groups. B cell epitope prediction limited immunogenicity to a few and small regions on c-TXNPx sequence. At cellular level, PBMC from asymptomatic and cardiac patients proliferated and secreted Interferon-γ after c-TXNPx stimulation, compared to Mock control. However, only proliferation was higher in asymptomatic patients compared to cardiac and non-infected subjects. Furthermore, asymptomatic patients showed an enhanced frequency of CD19 CD69 cells upon exposure to c-TXNPx. Overall, our results show that c-TXNPx fails to induce a strong immune response in natural infection, being measurable only in those patients without any clinical symptoms. The little impact of c-TXNPx in human immune response could be strategic for parasite survival, as it keeps this crucial anti-oxidant enzyme activity safe from the mechanisms of adaptive immune response. This article is protected by copyright. All rights reserved.

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Name: Immunology
ISSN: 1365-2567
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Medical and Biotech [MESH] Definitions

A hemoflagellate parasite affecting domestic and wild animals, as well as humans and invertebrates. Though it induces an immune response, it is non-pathogenic in humans and other vertebrates. It is cross-reactive with TRYPANOSOMA CRUZI and can thus cause false positives for CHAGAS DISEASE.

A genus of the subfamily TRIATOMINAE. Several species are vectors of TRYPANOSOMA CRUZI.

A genus of cone-nosed bugs of the subfamily TRIATOMINAE. Its species are vectors of TRYPANOSOMA CRUZI.

A genus of the subfamily TRIATOMINAE. Rhodnius prolixus is a vector for TRYPANOSOMA CRUZI.

The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.

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