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A Fluorescent Probe for Early Detection of Melanoma and Its Metas-tasis through Specifically Imaging Tyrosinase Activity in Mouse Model.

08:00 EDT 5th July 2018 | BioPortfolio

Summary of "A Fluorescent Probe for Early Detection of Melanoma and Its Metas-tasis through Specifically Imaging Tyrosinase Activity in Mouse Model."

Melanoma is a type of highly malignant and metastatic skin cancer, and early detection of melanoma by analyzing the level of its biomarker may decrease the likelihood of mortality. In this study, a fluorescent probe NBR-AP for detecting tyrosinase (a biomarker of melanoma) has been created by incorporating a hydroxyphenylurea group (as a substrate for the enzyme) onto a fluorescent dye phenoxazine derivative (as activatable signal reporter). This probe can be activated to generate fluorescence through a tyrosinase-mediated oxidation followed by hydrolysis of the urea linkage. The probe is able to sensitively and selectively detect the endogenous tyrosinase level in live cells and in zebrafish. Moreover, by imaging the tyrosinase activity, NBR-AP has been successfully applied to diagnose the melanoma and its metastasis in xenogeneic mouse models established via subcutaneous injection of B16F10cells.

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This article was published in the following journal.

Name: Analytical chemistry
ISSN: 1520-6882
Pages:

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