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Recombinant and native TviCATL from Trypanosoma vivax: enzymatic characterisation and evaluation as a diagnostic target for animal African trypanosomosis.

08:00 EDT 7th July 2018 | BioPortfolio

Summary of "Recombinant and native TviCATL from Trypanosoma vivax: enzymatic characterisation and evaluation as a diagnostic target for animal African trypanosomosis."

African animal trypanosomosis (nagana) is caused by tsetse-transmitted protozoan parasites. Their cysteine proteases are potential chemotherapeutic and diagnostic targets. The N-glycosylated catalytic domain of Trypanosoma vivax cathepsin L-like cysteine protease, rTviCATL, was recombinantly expressed and purified from culture supernatants while native TviCATL was purified from T. vivax Y486 parasite lysates. Typical of Clan CA, family C proteases, TviCATL activity is sensitive to E-64 and cystatin and substrate specificity is defined by the S pocket. Leucine was preferred in P and basic and non-bulky, hydrophobic residues accepted in P and P respectively. Reversible aldehyde inhibitors, antipain, chymostatin and leupeptin, with Arg in P and irreversible peptidyl chloromethylketone inhibitors with hydrophobic residues in P inhibited TviCATL activity. TviCATL digested host proteins: bovine haemoglobin, serum albumin, fibrinogen and denatured collagen (gelatine) over a wide pH range, including neutral to slightly acidic pH. The recombinant catalytic domain of TviCATL showed promise as a diagnostic target for detecting T. vivax infection in cattle in an indirect antibody detection ELISA.

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This article was published in the following journal.

Name: Molecular and biochemical parasitology
ISSN: 1872-9428
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Medical and Biotech [MESH] Definitions

A protozoan parasite that causes vivax malaria (MALARIA, VIVAX). This species is found almost everywhere malaria is endemic and is the only one that has a range extending into the temperate regions.

A protozoan parasite that occurs naturally in the macaque. It is similar to PLASMODIUM VIVAX and produces a type of malaria similar to vivax malaria (MALARIA, VIVAX). This species has been found to give rise to both natural and experimental human infections.

An active blood parasite that is present in practically all domestic animals in Africa, the West Indies, and parts of Central and South America. In Africa, the insect vector is the tsetse fly. In other countries, infection is by mechanical means indicating that the parasites have been introduced to these countries and have been able to maintain themselves in spite of the lack of a suitable intermediate host. It is a cause of nagana, the severity of which depends on the species affected.

Malaria caused by PLASMODIUM VIVAX. This form of malaria is less severe than MALARIA, FALCIPARUM, but there is a higher probability for relapses to occur. Febrile paroxysms often occur every other day.

A hemoflagellate parasite affecting domestic and wild animals, as well as humans and invertebrates. Though it induces an immune response, it is non-pathogenic in humans and other vertebrates. It is cross-reactive with TRYPANOSOMA CRUZI and can thus cause false positives for CHAGAS DISEASE.

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