Advertisement

Topics

Reciprocal enhancement of gene expression between the phz and prn operon in Pseudomonas chlororaphis G05.

08:00 EDT 11th July 2018 | BioPortfolio

Summary of "Reciprocal enhancement of gene expression between the phz and prn operon in Pseudomonas chlororaphis G05."

In previous studies with Pseudomonas chlororaphis G05, two operons (phzABCDEFG and prnABCD) were confirmed to respectively encode enzymes for biosynthesis of phenazine-1-carboxylic acid and pyrrolnitrin that mainly contributed to suppression of some fungal phytopathogens. Although some regulators were identified to govern their expression, it is not known how two operons coordinately interact. By constructing the phz- or/and prn- deletion mutants, we found that in comparison with the wild-type strain G05, phenazine-1-carboxylic acid production in the mutant G05Δprn obviously decreased in GA broth in the absence of prn, and pyrrolnitrin production in the mutant G05Δphz remarkably declined in the absence of phz. By generating the phzA and prnA transcriptional and translational fusions with a truncated lacZ on shuttle vector or on the chromosome, we found that expression of the phz or prn operon was correspondingly increased in the presence of the prn or phz operon at the post-transcriptional level, not at the transcriptional level. These results indicated that the presence of one operon would promote the expression of the other one operon between the phz and prn. This reciprocal enhancement would keep the strain G05 producing more different antifungal compounds coordinately and living better with growth suppression of other microorganisms.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of basic microbiology
ISSN: 1521-4028
Pages:

Links

DeepDyve research library

PubMed Articles [19260 Associated PubMed Articles listed on BioPortfolio]

Hydrogen cyanide produced by Pseudomonas chlororaphis O6 is a key aphicidal metabolite.

A biocontrol bacterium Pseudomonas chlororaphis O6 promotes plant health through multifaceted mechanisms. In this study, we used P. chlororaphis O6 mutants to examine metabolites with aphicidal activi...

Enhanced biosynthesis of arbutin by engineering shikimate pathway in Pseudomonas chlororaphis P3.

Arbutin is a plant-derived glycoside with potential antioxidant, antibacterial and anti-inflammatory activities. Currently, it is mainly produced by plant extraction or enzymatic processes, which suff...

Aminoglycoside-inducible expression of the mexAB-oprM multidrug efflux operon in Pseudomonas aeruginosa: Involvement of the envelope stress-responsive AmgRS two-component system.

Exposure of P. aeruginosa to the aminoglycoside (AG) paromomycin (PAR) induced expression of the PA3720-armR locus and the mexAB-oprM multidrug efflux operon that AmgR controls, although PAR induction...

The nitrogen regulator PipX acts in cis to prevent operon polarity.

Cyanobacteria, phototrophic organisms performing oxygenic photosynthesis, must adapt their metabolic processes to important environmental challenges, like those imposed by the succession of days and n...

The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37.

To cause disease, Clostridioides (Clostridium) difficile must resist killing by innate immune effectors in the intestine, including the host antimicrobial peptide, cathelicidin (LL-37). The mechanisms...

Clinical Trials [3597 Associated Clinical Trials listed on BioPortfolio]

The Gene Expression Studies of the Role of Tumor Microenvironments in Tumor Progression

The purpose of this study is to analyze the gene expression patterns associated with various microenvironmental stresses in tumors to understand their roles in tumor progression and treatm...

Gene Expression Profiles in Chronic Periodontitis

Current concepts of the causes of periodontitis implicate a bacterial infection as the primary cause of the disease. Several bacterial species residing in a biofilm on tooth surfaces are c...

DNA Microarray Analysis of Gene Expression in Chronic Rhinosinusitis

Analysis of differential gene expression in patients with chronic rhinosinusitis without polyps compared to unaffected patients. Correlation of abnormal gene expression with surgical outco...

Investigation of the HDAC4 Copy Number Variation and Its Effect on Gene and Protein Expression in Patients With ASD

Specific Aims of this study: 1. To understand the distribution of HDAC4 CNV in the families of the ASD patients with HDAC4 CNV; 2. To perform the analysis of gene expressi...

Construction and Use of Lung Tumor Microarray for the Analysis of Gene Expression in Lung Cancer

The proposed research intends to construct a set of tissue microarrays containing different types of normal and lung cancer tissues for the study of genes associated with lung cancer. Thus...

Medical and Biotech [MESH] Definitions

A non-pathogenic species of Pseudomonas that occurs in soil, especially in the RHIZOSPHERE surrounding plant roots, as well as on the leaves of plants. It has anti-fungal and anti-nematode activities and is used in agriculture as a biocontrol agent.

Regulatory genes which encode a cyclic AMP receptor protein required for L-arabinose utilization in E. coli. It is an example of positive control or regulation of gene expression in the bacterial operon.

The expression of a gene in an abnormal place, or at an abnormal time in an organism. Ectopic Gene Expression is often induced artificially by genetic techniques.

A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.

A non-metabolizable galactose analog that induces expression of the LAC OPERON.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Gene Expression
The process of gene expression is used by eukaryotes, prokaryotes, and viruses to generate the macromolecular machinery for life. Steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-tran...

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...


Searches Linking to this Article