Direct conversion of cellulose into ethanol and ethyl-β-d-glucoside via engineered Saccharomyces cerevisiae.

08:00 EDT 16th July 2018 | BioPortfolio

Summary of "Direct conversion of cellulose into ethanol and ethyl-β-d-glucoside via engineered Saccharomyces cerevisiae."

Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio-degradable surfactant, ethyl-β-d-glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT-1) and intracellular β-glucosidase (GH1-1) originating from Neurospora crassa. We identified the formation of ethyl-β-d-glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1-1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl-β-d-glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g/L/h, respectively, during the SSF of Avicel PH-101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co-production of a value-added chemical (alkyl-glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1-1 in engineered S. cerevisiae. This co-production could have a substantial effect on overall techno-economic feasibility of SSF of cellulose. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: Biotechnology and bioengineering
ISSN: 1097-0290


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