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Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio-degradable surfactant, ethyl-β-d-glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT-1) and intracellular β-glucosidase (GH1-1) originating from Neurospora crassa. We identified the formation of ethyl-β-d-glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1-1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl-β-d-glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g/L/h, respectively, during the SSF of Avicel PH-101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co-production of a value-added chemical (alkyl-glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1-1 in engineered S. cerevisiae. This co-production could have a substantial effect on overall techno-economic feasibility of SSF of cellulose. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Biotechnology and bioengineering
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A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A gas that condenses under slight pressure. Because of its low boiling point ethyl chloride sprayed on skin produces an intense cold by evaporation. Cold blocks nerve conduction. Ethyl chloride has been used in surgery but is primarily used to relieve local pain in sports medicine.
Cellulose derivative used in chromatography, as ion-exchange material, and for various industrial applications.
Organic compounds having ethyl groups bound to an oxygen atom