Bromelain Kinetics and Mechanism on Myofibril from Golden Pomfret (Trachinotus blochii).

08:00 EDT 18th July 2018 | BioPortfolio

Summary of "Bromelain Kinetics and Mechanism on Myofibril from Golden Pomfret (Trachinotus blochii)."

Bromelain was used to tenderize golden pomfrets (Trachinotus blochii). The enzyme kinetic model was x=2.447×ln[1+(1332.21×E0S0-1.74)t], which indicated that the degree of hydrolysis (DH, x) was dependent on hydrolysis time (t), the initial concentration of myofibril (S ) and bromelain (E ). The relationship between the overall hydrolysis rate (v), S , E , and t is demonstrated as: v=(16.50(E0S0)-1.33)S0 exp {-2.447ln[1+(1332.21E0S0-1.74)t2]}. Sample of 0.40% E /S was further used to study the effects of hydrolysis time on the changes of proteins, peptides, free amino acids (FAA), and protein nanostructure. SDS-PAGE result showed that myosin heavy chain was degraded dramatically from 22.88% before treatment to 12.03% after 2 min bromelain treatment. Meanwhile, bromelain did not exhibit activity towards actin, trypomyosin, myosin light chain, and troponin C. A general increase of amino acids indicated the increased DH and the preferential cleavage sites of bromelain in the descending order of lysine, glutamic acid, glycine, ornithine, methionine sulfoxide, and alanine. Atomic force microscope images showed that the strip-like structure of myofibril was considerably degraded by bromelain, and the granulation of protein after 20 min indicated possible self-assembling of protein hydrolysate. Confocal laser scanning microscopy further confirmed the degradation of myofibril proteins and formation of protein aggregates.


Journal Details

This article was published in the following journal.

Name: Journal of food science
ISSN: 1750-3841


DeepDyve research library

PubMed Articles [7129 Associated PubMed Articles listed on BioPortfolio]

Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus.

Bromelain, a protease from pineapple plant can be applied as oral drug for the treatment of inflammation and certain diseases. Unlike most conventional supports, immobilization on edible support will ...

Long-chain polyunsaturated fatty acid biosynthesis and its response to cadmium exposure in silver pomfret.

Despite a close interaction between cadmium (Cd) and long-chain polyunsaturated fatty acid (LC-PUFA) metabolism, the influence of Cd exposure on the endogenous synthesis of LC-PUFA has received little...

Enkephalin related peptides are released from jejum wall by orally ingested bromelain.

Stem bromelain [EC] is a thiol-endopeptidase and orally recommended in traditional medicine due to its analgesic activity, but the mechanisms are not known. Proenkephalin is expressed in the...

Bromelain inhibits the ability of colorectal cancer cells to proliferate via activation of ROS production and autophagy.

Advanced colorectal cancer (CRC) survival rates are still low despite advances in cytotoxic and targeted therapies. The development of new effective or alternative therapies is therefore urgently need...

Bromelain with peroxidase from pineapple are more potent to target leukemia growth inhibition - A comparison with only bromelain.

The natural anti-cancer agent bromelain is found to be beneficial for either single or multi-targeted therapy in gastric and skin carcinoma, by inhibiting cancer cell growth. Importantly, the presence...

Clinical Trials [1618 Associated Clinical Trials listed on BioPortfolio]

Arnica Montana and Bromelain in Rhinoplasty

The purpose of this study is to assess the perioperative use of Arnica Montana and the combined use of Arnica Montana and Bromelain in aiding the body's wound healing functions during the ...

Bioavailability of Golden Rice Carotenoids in Humans

This investigation protocol uses an isotope reference method to evaluate bioavailability of ß-C in rice and its bioconversion to vitamin A. A well-nourished population will be fed hydrop...

Golden Black Seed: Support of Metabolic Health and General Wellness.

To document effects of consuming Golden Black Seed (brand: New Chapter Inc.) on metabolic health and wellness in human subjects. Golden Black Seed contains extracts from the turmeric root...

Glucose Kinetics After Wheat and Rye Breads

Certain functional properties of cereal products, e.g. their postprandial glucose and insulin responses, have been characterized as steps towards obtaining a greater understanding of their...

Influence of Vorapaxar on Thrombin Generation and Coagulability

Currently, there are no data available regarding the effect of vorapaxar on clot generation kinetics or TIP-FCS when added to standard of care antiplatelet regimens. Potential reduction of...

Medical and Biotech [MESH] Definitions

Protein-digesting and milk-clotting enzymes found in PINEAPPLE fruit juice and stem tissue. Enzymes from the two sources are distinguished as fruit bromelain and stem bromelain. There has been interest in using it for INFLAMMATION and DEBRIDEMENT. This enzyme was formerly listed as EC

A family of Primates of the suborder Strepsirhini containing six genera. The family is distributed in parts of Africa, India, Asia, and the Philippines. The six genera are: Arctocebus (golden potto), GALAGO (bush babies), Loris (slender loris), Nycticebus (slow loris), and Perodicticus (potto). Lorises and pottos are relatively common except for Arctocebus, the golden potto. All are arboreal and nocturnal.

The repeating contractile units of the MYOFIBRIL, delimited by Z bands along its length.

A genus of GOLDEN-BROWN ALGAE in the family Ochromonadaceae, found mostly in freshwater. They bear two unequal FLAGELLA and are heterotrophic.

A family of microscopic freshwater EUKARYOTA, commonly known as golden algae. They share many features with the BROWN ALGAE but are planktonic rather than benthic. Though most are photosynthetic, they are not considered truly autotrophic since they can become facultatively heterotrophic in the absence of adequate light. In this state they can feed on BACTERIA or DIATOMS.

Quick Search


DeepDyve research library

Relevant Topics

Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article