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In this study, a cloned almA gene was inserted into two plasmids PUC57 and pet-28a (+), and then transformed into Escherichia coli competent strains. The presence of almA was confirmed by PCR combined with restriction enzyme digestion. The results indicated two strains of PUC57/almA-E. coli and pet-28a (+)/almA-E. coli were all functioned on biodegradation long-chain alkanes, however, pet-28a (+)/almA-E. coli strain performed better and the speed of biodegradation was enhanced to about 12 mg L h when compared with PUC57/almA-E. coli strain. Furthermore, degradation degree of n-C32 was much higher than other long-chain alkanes for two engineered E. coli strains. Our results further suggested that exoenzyme was only detected in pet-28a (+)/almA-E. coli and followed a decreasing order of extracellular enzyme > intracellular enzyme > periplasmic enzyme. Besides, co-biodegradation of petroleum hydrocarbons by Pseudomonas sp. B-1, Bacillus subtilis PH-1 with exoenzyme or pet-28a (+)/almA-E. coli posed enormous potential that could reach 70%-80% in oil pollution bioremediation.
This article was published in the following journal.
Name: International journal of biological macromolecules
The Algerian coastline is exposed to several types of pollution, including hydrocarbons. The aim of this work was to isolate oil-degrading bacteria and to explore the intrinsic bioremediation potentia...
Microbes appear to play a key role in bioremediation of petroleum hydrocarbons pollution and little attention has been paid to the enzyme activity in the process of alkane bioremediation. Oil field ba...
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The thermophilic anaerobic co-digestion of food waste and paper waste was successfully operated with a 0% to 70% fraction of paper waste. The variation of functional microbial community was investigat...
TMC435-TiDP16-C107: This Study Measures the (Possible) Influence of TMC435 on the Activity of a Selected Set of Drug-degrading Proteins by Measuring the Blood Levels of Drugs That Have Been Taken Together With TMC435 and That Are Known to be Specifically
The purpose of this study is to determine whether TMC435 influences the activity of certain drug-degrading proteins in the human body. The drug-degrading proteins investigated in this stud...
Humans eat long-chain fat in their diet and use it for energy during exercise and during periods of fasting. Patients with long-chain fatty acid oxidation disorders cannot use dietary fat ...
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A flavoprotein oxidoreductase that has specificity for long-chain fatty acids. It forms a complex with ELECTRON-TRANSFERRING FLAVOPROTEINS and conveys reducing equivalents to UBIQUINONE.
A long-chain fatty acid ester of carnitine which facilitates the transfer of long-chain fatty acids from cytoplasm into mitochondria during the oxidation of fatty acids.
A mitochondrial protein consisting of four alpha-subunits and four beta-subunits. It contains enoyl-CoA hydratase, long-chain-3-hydroxyacyl-CoA dehydrogenase, and acetyl-CoA C-acyltransferase activities and plays an important role in the metabolism of long chain FATTY ACIDS.
An ATP-Binding Cassette Transporter that functions in the import of long chain (13-21 carbons) and very long chain fatty acids (> 22 carbons), or their acyl-CoA-derivatives, into PEROXISOMES. Mutations in the ABCD1 gene are associated with the X-linked form of ADRENOLEUKODYSTROPHY.
Funding resources and procedures for capital improvement or the construction of facilities.
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...