Advertisement

Topics

Efficient UPLC and CE Methods for the Simultaneous Determination of Azelastine Hydrochloride and Its Genotoxic Impurity.

08:00 EDT 25th July 2018 | BioPortfolio

Summary of "Efficient UPLC and CE Methods for the Simultaneous Determination of Azelastine Hydrochloride and Its Genotoxic Impurity."

A novel Stability indicating UPLC and CE methods were established and validated for the determination of azelastine hydrochloride (AZL) and its genotoxic impurity, benzohydrazide (BHZ) in the presence of benzalkonium chloride (BC). The developed UPLC method was based on chromatographic separation using C18 column as a stationary phase and acetonitrile: (0.1 % w/v) aqueous sodium lauryl sulfate (55:45, v/v, pH=5 with phosphoric acid) as a mobile phase with a flow rate of 1.2 mL/min and UV detection at 215 nm. The chromatographic run time was approximately 2 min. On the other hand, the developed CE method depended on using a stationary phase of Standard Bare Fused Silica Capillaries (75 μm i.d. × 59 cm and 50 cm detection length) and the applied voltage was 30 KV using 40 mM Phosphate buffer (pH=2 with aqueous H PO ), the detection wave length was at 225 nm. Analysis time was about 6 min. The suggested methods were successfully applied for the analysis of AZL in pharmaceutical preparation. The validity of the developed methods was assessed by applying the standard addition technique and no interference from excipients was observed. The results obtained by the proposed methods were statistically analyzed and compared with the manufacturer's method and no significant difference was found between the compared methods.

Affiliation

Journal Details

This article was published in the following journal.

Name: Biomedical chromatography : BMC
ISSN: 1099-0801
Pages: e4346

Links

DeepDyve research library

PubMed Articles [14641 Associated PubMed Articles listed on BioPortfolio]

Efficacy of a Novel Intranasal Formulation of Azelastine Hydrochloride and Fluticasone Propionate, Delivered in a Single Spray, for the Treatment of Seasonal Allergic Rhinitis: Results from Russia.

The novel intranasal formulation of azelastine hydrochloride (AZE) and fluticasone propionate (FP) in a single spray (MP-AzeFlu) was compared with a first-line intranasal antihistamine spray (AZE) in ...

UPLC-MS/MS method for the simultaneous determination of imatinib, voriconazole and their metabolites concentrations in rat plasma.

In the present study, a simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method used to measure the plasma concentrations of imatinib, voriconazole and their metabo...

Modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes for simultaneous electrochemical determination of biocompounds in biological fluids.

In this study, a simple modified glassy carbon electrode with Polydopamine-multiwalled carbon nanotubes (GCE/PDA-MWCNTs) is described for selective and sensitive simultaneous voltammetric determinatio...

Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies.

DNA double-strand breaks (DSBs), one of the most severe lesions of DNA damage triggered by various genotoxic insults, can lead to chromosome change, genomic instability, and even tumorigenesis if not ...

Simultaneous ultraperformance liquid chromatography/tandem mass spectrometry determination of four antihypertensive drugs in human plasma using hydrophilic-lipophilic balanced reversed phase sorbents sample preparation protocol.

Therapeutic drug monitoring of angiotensin converting enzyme inhibitors (ACEI) has a great impact in blood pressure control in patient with heart failure, hepatic and renal impairment. To provide an e...

Clinical Trials [5198 Associated Clinical Trials listed on BioPortfolio]

A Study to Evaluate the Safety and Effectiveness of a Nasal Spray to Treat Seasonal Allergies

The purpose of this study is to determine if two allergy medications (azelastine and fluticasone) are more effective than placebo or either medication alone (azelastine or fluticasone)

A Study to Evaluate the Safety and Effectiveness of a Nasal Spray to Treat Seasonal Allergies

The purpose of this study is to determine if two allergy medications (azelastine and fluticasone) are more effective than placebo or either medication alone (azelastine or fluticasone)

A Study to Evaluate the Safety and Effectiveness of a Nasal Spray to Treat Seasonal Allergies

The purpose of this study is to determine if the combination of two allergy medications (formulated azelastine/fluticasone product)is more effective than placebo or either component medica...

Study to Evaluate the Safety and Tolerability of a Nasal Spray in Patients With Chronic Allergic or Nonallergic Rhinitis

The purpose of this study is to determine if Astepro Nasal Spray (0.1% azelastine hydrochloride) is as safe as Astelin Nasal Spray (0.1% azelastine hydrochloride)

A Study to Evaluate the Safety and Effectiveness of a Nasal Spray to Treat Seasonal Allergies

The Purpose of this study is to determine if one allergy medication (0.15% azelastine hydrochloride) is more effective than Placebo alone.

Medical and Biotech [MESH] Definitions

Methods for controlling genetic SEX of offspring. It may include SEX DETERMINATION (GENETICS) and other means.

The simultaneous, or near simultaneous, transference of heart and lungs from one human or animal to another.

Simultaneous infection of a host organism by two or more pathogens. In virology, coinfection commonly refers to simultaneous infection of a single cell by two or more different viruses.

Utilization of all available receptive and expressive modes for the purpose of achieving communication with the hearing impaired, such as gestures, postures, facial expression, types of voice, formal speech and non-speech systems, and simultaneous communication.

A pharmaceutical preparation of sitagliptin phosphate and metformin hydrochloride that is used in the treatment of TYPE 2 DIABETES.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...


Searches Linking to this Article