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Little is known of the occurrence and age patterns of species/genotypes and subtypes of Cryptosporidium spp. and Giardia duodenalis in calves in Egypt. In this study, 248 fecal specimens were collected from dairy calves aged 1 day to 6 months on eight farms in three provinces during March 2015 to April 2016. Cryptosporidium spp. were detected and genotyped by using PCR-RFLP analysis of the small subunit rRNA (SSU rRNA) gene, while G. duodenalis was detected and genotyped by using PCR and sequence analyses of the triose phosphate isomerase (tpi), glutamate dehydrogenase (gdh) and β-giardin (bg) genes. The overall infection rates of Cryptosporidium spp. and G. duodenalis were 9.7 and 13.3%, respectively. The highest Cryptosporidium infection rate (26.7%) was in calves of age ≤ 1 month while the highest G. duodenalis infection rate (44.4%) was in calves of 2 months. Three Cryptosporidium spp. were identified, including C. parvum (n = 16), C. bovis (n = 5) and C. ryanae (n = 3), with the former being almost exclusively found in calves of ≤3 months of age and the latter two being only found in calves of over 3 months. Subtyping of C. parvum by PCR-sequence analysis of the 60 kDa glycoprotein gene identified subtypes IIaA15G1R1 (n = 15) and IIaA15G2R1 (n = 1). The G. duodenalis identified included both assemblages E (n = 32) and A (n = 1), with the latter belonging to the anthroponotic subtype A2. These data provide new insights into the genetic diversity and age patterns of Cryptosporidium spp. and G. duodenalis in calves in Egypt.
This article was published in the following journal.
Name: Parasitology international
Giardia duodenalis and Cryptosporidium spp. are common gastrointestinal protozoa in mammals. Many studies have been conducted on the distribution of G. duodenalis and Cryptosporidium spp. genotypes in...
Giardia duodenalis is a gastrointestinal parasitic protozoan with important veterinary and public health implications worldwide. In this study, a total of 1440 fecal specimens were collected from dair...
The purpose of this study was to monitor the presence of Cryptosporidium spp. and Giardia duodenalis in a water treatment plant (WTP) using settling sludge and backwash water (BW) samples in previous ...
The objective of this study was to describe preweaned dairy heifer calf management practices on dairy operations across the United States that were used to analyze factors associated with colostrum qu...
Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are globally ubiquitous pathogens. However, little is known about the occurrence and distribution of Cryptosporidium spp., G. duod...
A Randomized, Multicenter, Double-Blind, Parallel-Group, Placebo-Controlled Trial of the Efficacy and Safety of Bovine Anti-Cryptosporidium Immunoglobulin (BACI) in the Treatment of Cryptosporidium Enteritis in AIDS Patients
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A civilization of ancient Northeastern Africa, concentrated along the lower reaches of the Nile River in the area of Egypt. It was active from 3100 B.C. until its conquest by Alexander the Great in 332 B.C.
A species of VARICELLOVIRUS that causes a fatal MENINGOENCEPHALITIS in calves.
A species of parasitic protozoa that infects humans and most domestic mammals. Its oocysts measure five microns in diameter. These organisms exhibit alternating cycles of sexual and asexual reproduction.
Polymerase Chain Reaction (PCR)
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