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Closed genome and comparative phylogenetic analysis of the clinical multidrug resistant Shigella sonnei strain 866.

08:00 EDT 30th July 2018 | BioPortfolio

Summary of "Closed genome and comparative phylogenetic analysis of the clinical multidrug resistant Shigella sonnei strain 866."

Shigella sonnei is responsible for the majority of shigellosis infections in the United States with over 500,000 cases reported annually. Here we present the complete genome of the clinical multidrug resistant (MDR) strain 866, which is highly susceptible to bacteriophage infections. The strain has a circular chromosome of 4.85 Mb and carries a 113 kb MDR plasmid. This IncB/O/K/Z-type plasmid, termed p866, confers resistance to five different classes of antibiotics including ß-lactamase, sulphonamide, tetracycline, aminoglycoside, and trimethoprim. Comparative analysis of the plasmid architecture and gene inventory revealed that p866 shares its plasmid backbone with previously described IncB/O/K/Z -type Shigella spp. and E. coli plasmids, but is differentiated by the insertion of antibiotic resistance (AR) cassettes, which we found associated with mobile genetic elements such as Tn3, Tn7, and Tn10. A whole genome-derived phylogenetic reconstruction showed the evolutionary relationships of S. sonnei strain 866 and the four established Shigella species, highlighting the clonal nature of S. sonnei.

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Name: Genome biology and evolution
ISSN: 1759-6653
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